NMR Core Facility Training Course

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NMR Core
Facility Training Course
High-Field Biomacromolecular Solution NMR Core
Facility National
Research Program for Genomic Medicine Topics
Protein Solution NMR Spectroscopy. Instructor
Tai-huang Huang, (Chi-Fon Chang and Winston
Wu) Time  Every Saturday 1000 AM - 100 PM
for 15 sessions (First
session Oct 2, 2004) Place B1A room,
IBMS, Academia Sinica Pre-requisite Some prior
experience with protein NMR spectroscopy.
Textbooks 1. Lecture by James Keeler on
Understanding NMR spectroscopy
(http//www-keeler.ch.cam.ac.uk/lectures/)
2. Cavanagh, Fairbrother, Palmer, and
Skelton Protein NMR spectroscopy
Principles and practice Academic press, 1996.
3. Selected review articles.
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• Curse Content
• This will be a comprehensive lecture
  course, focusing on modern high field
• NMR spectroscopy in solution, with applications
  to protein structure, dynamics
• and functional studies. Topics to be covered
  include
• Basic NMR theory, including quantum mechanical
  and vectorial descriptions 
• of NMR spectroscopy.
• 2. Basic experimental aspects of NMR NMR data
  acquisition and processing.
• 3. Product operator formalism analysis of pulse
  programs.
• 3. Spin dynamics Coherent selection, phase
  cycling, gradient enhanced
• spectroscopy.
• 4. Heteronuclear multidimensional NMR
  spectroscopy.
• 5. Relaxation and protein dynamics.
• 6. Special topics TROSY, RDC and reduced
  dimensionality etc.
• 7. Applications in protein NMR in solution.

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• Course Outline
• Lect Date Topics
• 1 10/2 NMR and Energy level
• 2 10/9 Vector Model
• 3 10/16 Fourier Transform and
  Data processing
• 4 10/23 How the spectrometer works
• 5 11/20 Product Operator
• 6 11/27 Two dimensional NMR
• 7 12/4 Relaxation
• 8 12/11 Coherence selection and phase
  cycling
• 9 12/18
• 10 12/25
• 11 1/8/05
• 12 2/19/05
• 13 2/26/05
• 14 3/5/05
• 15 3/12/05

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NMR Historic Review
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2002 Nobel prize in Chemistry was awarded to
Kurt Wuthrich
NMR is a versatile tool and it has applications
in wide varieties of subjects in addition to its
chemical and biomedical applications, including
material and quantum computing.
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Felix Bloch 1952, Physics
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800 MHz
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Dominant interactions H HZ HD HS
HQ.HZ Zeeman Interaction HD
Dipolar Interactions HS Chemical Shielding
Interaction. HQ Quadrupolar Interaction

• Basic Nuclear Spin Interactions

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Electrons
3
3
Nuclear Spin j
Ho
Nuclear Spin i
Ho
1
1
2
5
4
4
Phonons
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