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Vietnam National University-HCM City University of Natural Sciences

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Transformation in plant using Agrobacterium tumefaciens and biolistic methods. . Transgenic plants in laboratory (e.g. Tobacco, Citrus, Cassava, Vetiver). . – PowerPoint PPT presentation

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Title: Vietnam National University-HCM City University of Natural Sciences


1
Vietnam National University-HCM City University
of Natural Sciences
  • Technology transfer Experiences in Department
    of Plant biotechnology Biotransformation
  • Associate Prof.Dr. Bui Van Le

2
Outline of presentation
  • Introduction
  • Current researches in plant biotechnology
  • Results in technology transfer
  • Some successful examples in technology transfer
  • Conclusions

3
Introduction
  • General researches of Universities
  • Basic scientists often make remarkable
    discoveries.
  • Fundamental researches.
  • Many Universities have made researches under
    governmental finance.
  • Lack of the co-operation with Industry.

4
  • Some Universities have technology transfer
    programs
  • - Research can directly benefit the public.
  • - Facilitating the commercialization of research
    results for the public.
  • Faculties have come to expect support for
    patenting and started businesses.
  • Some Universities make lots of money.

5

What are we doing now in plant Biotechnology?
  • Develop methods of Tissue culture for mass
    propagation of plant and metabolite secondary
    productions.
  • Develop methods of gene transfer in plant to
    monitor the dispersal of transgenic plants.
  • Utilize marker molecular to monitor plant
    resources and plant diseases.

6
Results in plant tissue culture
  • . Basic researches on model plants such as
    tobacco, Arabidopsis to study morphological
    program of plant cells using Thin Cell layers
    technique.
  • . Mass propagation of plants (e.g. Paulownia,
    Pinus carribea, Vetiver Zizanoids, Orchids,
    Saintpaulia..).
  • . Producing in vitro secondary metabolite
    compounds (e.g. Naphthoquinone in Drosera burmani
    Vahl).
  • ? Exploitation of plant propagation and
    natural product.

7
Results in plant transformation
  • . Transformation in plant using Agrobacterium
    tumefaciens and biolistic methods.
  • . Transgenic plants in laboratory (e.g. Tobacco,
    Citrus, Cassava, Vetiver).
  • . Develop some methods (PCR, Real time PCR) to
    detect transgenic plants based on target genes,
    promoter (CAMV35S) and terminator (tNOS)
  • ?Exploitation of detection transgenic plants in
    crop plants and food.

8
Results in plant resources
  • . Identification plant sources using maker
    molecular techniques such as RAPD (e.g. tomato,
    bananas)
  • . Detection plant diseases using PCR technique
    (e.g. Phytophthora infectans in tomato .)
  • . Study hybridization between species to obtain
    novel varieties (e.g. orchids)
  • ? Exploitation of plant breeding project and
    detection plant diseases.

9
Why do we use Technology Transfer Programs?
  • The processes of laboratory researches are
    transferred to organizations for development and
    commercialization.
  • Extend the parameters of technological innovation
    by using fundamental knowledge through focusing
    applied research and development.
  • - Participate in the establishment of technology
    and business.

10
Barriers
  • . Lack of highly professional management and
    mechanism of supporting .
  • . Lack of appropriate resources to approach
    up-date information and find out the
    opportunities for technology cooperation.
  • . Short term of production.

11
Some successful examples in technology transfer
  • Technology transfer in Saintpaulia ionantha.
  • Technology transfer in orchids.
  • Technology transfer in molecular methods.

12
In case of study in Saintpaulia species.
  • Technological issues
  • Produced in the world.
  • Little information about technological
    productions (only by cutting).
  • Research issues
  • Educational programs (plant tissue culture).
  • Facilities for mass propagation.
  • Effective applications of the process
  • Novel plants.
  • Cycle productions 4-5 months.

13
Technology transfer strategies for Saintpaulia
  • The establishment of plant tissue culture system
    about the innovation and technology transfer for
    students
  • Integrating education and RD programs
  • The development of appropriate innovations,
    technology transfer models and support systems

14
Results in Saintpaulia ionantha
  • Produce 75.000 plants in 3 years.
  • Transfer technique in local sectors.

15
In case of study in orchids.
  • Technological issue
  • Produced in the world.
  • Much information about technological productions
    (by tissue culture).
  • Research issue.
  • Research programs (tissue culture).
  • Effective application of process.
  • Low technical capability.
  • Cycle productions 1-5 years.

16
Technology transfer strategies for orchids
  • Combination with individual organizations for
    Innovation and Technology Transfer.
  • The development of appropriate innovations,
    technology transfer models and support systems.

17
Results in technology transfer for orchids
  • Produce 100.000 plants in 2 years

18
In case of study in plant marker molecular
  • Technological issue
  • Useful techniques in the world.
  • Much information about technique.
  • Research issue.
  • Research programs.
  • Effective application of the process.
  • Highly technical capability.
  • Fast detections.

19
Technology transfer strategies for plant marker
molecular
  • Exchange experiences, models and the best
    practices through networks.
  • Standardization Innovation and Technology
    Transfer.

20
Results in technology transfer for plant marker
molecular
  • Methods of detection plant diseases.
  • Methods of detection GMOs.
  • Methods of identification plant breeding.

21
CONCLUSIONS
  • Results of researches in universities start the
    technology transfer processes.
  • Enhancement RD program at Universities.
  • Its necessary to have a support mechanism for
    technology transfer.
  • Universities play professionally consultative
    role.

22
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