????-1: Methods in Molecular Medicine

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????-1 Methods in Molecular Medicine

• ???
• zhangxianning_at_zju.edu.cn
• Tel[phone] 88208367
• Office A705, Research Building
• 2013/09

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Trends of Medicine in 21th Century

• Treatment afterward ?Predictive Preventive

• Molecular epidemiology Sub-population

• Rare diseases ? Common diseases

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• Why Clone Human Genes?
• To define inherited genetic mutations
• Develop diagnostic reagents (pre-natal testing)
• Develop targeted therapies
• To isolate functional/normal genes
• Prepare normal protein products (Factor VIII)
• Therapeutic use of proteins normally produced at
  low levels
• Vaccine development (avoid attenuated organisms)
• To isolate genes with somatic mutations
• Develop diagnostic reagents
• Target therapy to altered gene product
• To define and understand cellular pathways and
  circuits
• Signal transduction, immunity, development, cell
  cycle and apoptosis

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The basic tools of gene exploration

• Restriction-enzyme analysis