???(Arabidopsis thaliana)???????,??????(125 Mbp)?????????,???????????(The Arabidopsis Genomic Initiative, 2000)?

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???(Arabidopsis thaliana)???????,??????(125
Mbp)?????????,???????????(The Arabidopsis Genomic
Initiative, 2000)?
?????????,????????????????????????? ??????????????
??????????????? ?????????????????????????????
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????(map-based cloning)??????(positional
cloning),1986?????????Alan Coulson??,?????????????
?????????????,?????????DNA??,?????????????????????
????????????????????????????????
???????,????????(Col-0???)????????????????????????
?????????(?1)?
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???? ??????????????????????????? ??????????DNA???
?????????,????????????????????? ????,?????????????
????,?????????????????(SSLP),????????(SNP)?
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SSLP???????????PCR?????,?????????????(?Col?Ler???
???,?1000bp?411??????),???????,????????,?????????
???SSLP??
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SNP???????,?????????????????????????,????????????
?????? ????????SNP??????????(??)???????(CAPS)
,?????PCR??
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??????????????????????,?????????????????Col-0?Ler?
?????????,?????????????????????????, ??????????,??
????????????(Col-0??Ler)??????????F1?????F1???????
??,?????????????????????F1????????????????????????
???????????
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??
?? ?????????????
col0
??
Landsberg???
?????

?????
?????????????????
10
??????
???????,??????????????????????????
1 2 3 4 5
1 2 3 4 5

Landsberg???
Col-0???
A
a
a
A
???
F1
a
A

F2
A
A
a
A
a
A
a
a
11
maker
a
a
a
a
a
a
a
a
???
???
???
???
Col-0 Ler
Col-0 Ler
Col-0
Ler
???2???
???
100
2????
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?15?maker?,?????????????
???
a
?????
???maker
???maker
???maker
?3?maker?,????????????maker??
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?????????????????40 Kb????????????,???????????????
??????????PCR????????40 Kb??????500
bp?????????????????????40 Kb???,??????????(Col-0??
Ler)???????,????????????????? ????????????????????
?????????????????????????????????BAC???YAC?????cDN
A??,??????????,????????,????????????????????(1)??
?????cDNA??????????(2)??cDNA?????????????(3)??cD
NA??,?????,?????????(4)???????,??DNA?????????????
(5)????????,??????? ????????????????????????
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?????
?????????????,??????,????????????????????
1.????????????????????? 2.??(??)???????????????
??????,????DNA?????,??????????????????? 3.????????
????????????????,???????????????,1??????????100--
400 Kb?????,???250 Kb????????????????,???1???????
???1000--2500 Kb?