Ligation Chart

1
Ligation Chart
FOR Stage 1 Stage 2 Stage 3
J37015 C0261 (Vector) I13504 (Insert) PCR Product 442bp F2620 (Vector) (C0261 I13504) (Insert) PCR Product 442bp Finalised Prey Cell
J37015 Riboswitch Riboswitch (Insert) (C0261 I13504) (Vector) PCR Product F2620 (Vector) (RS C0261 I13504) (Insert) PCR Product 442bp Issues Talk to john SY Finalised Prey Cell with Riboswitch
J37015 LoxP Lox Sites (UNKNOWN) (C0261 I13504) (UNKNOWN) PCR Product F2620 (Vector) (LOX C0261 I13504) (Insert) PCR Product 442bp Finalised Prey Cell with Lox Sites
J37016 J37019 (Vector) I13504 (Insert) Monocistronic Predator Test Construct
J37019 B0034 (Vector) C0062 (Insert) PCR Product 527bp R0062 (Vector) (B0034 C0062) (Insert) PCR Product 527bp
J37020 I13033 (Vector) I13504 (Insert) PCR Product 434bp J37019 (Vector) (I13033 I13504) (Insert) PCR Product 265bp Polycistronic Predator Test Construct
J37025A B0034 (Vector) J37024 (Insert) AiiA For Predator
J37024A J37023 (Insert) B0015 (Vector)
J37022A J04500 (Vector) J37024 (Insert) AiiA Test Construct
Cre J04500 (Vector) Cre (Insert) (J04500 Cre) (Insert) B0015 (Vector) Cre - required for finalised prey cell with LoxP sites

Final Ligation J37019 (Vector) J37025 (Insert)


C0261 (24A) Cut S P Primer pSB1(A2/A3/AK3)
I13504 (12D) Cut X P Primer I13504
C0261 I13504 Cut X P Primer I13504
F2620 (6B) Cut S P Primer pSB1(A2/A3/AK3)
C0261 I13504 Cut E X Primer pSB1A2 Primer
(Reverse)
Riboswitch Cut Pre-cut Primer Riboswitch
RS C0261 I13504 Cut X P Primer I13504
F2620 (6B) Cut S P Primer pSB1(A2/A3/AK3)
Depends on PCR which is yet to work in our lab
C0261 I13504 Cut UNKNOWN PrimerUNKNOWN
Lox Sites Cut UNKNOWN Primer UNKNOWN
LOX C0261 I13504 Cut X P Primer I13504
F2620 (6B) Cut S P Primer pSB1(A2/A3/AK3)
J37019 Cut S P Primer pSB1(A2/A3/AK3)
I13504 Cut X P Primer I13504
B0034 (3O) Cut S P Primer pSB1(A2/A3/AK3)
C0062 (7A) Cut X P Primer C0062 2
R0062 (9G) Cut S P Primer pSB1(A2/A3/AK3)
B0034 C0062 Cut X P Primer C0062 2
I13033 (2H) Cut S P Primer pSB1(A2/A3/AK3)
I13504 (12D) Cut X P Primer I13504
I13033 I13504 Cut X P Primer I13504
J37019 Cut S P Primer pSB1(A2/A3/AK3)
B0034 (3O) Cut S P Primer pSB1(A2/A3/AK3)
J37024 Cut X P Primer UNKNOWN
Waiting on immuno tag
J37023 Cut S E Primer UNKNOWN
B0015 (1I) Cut X E Primer UNKNOWN
Depends on PCR which is yet to work in our lab
J04500 (16P) Cut S P Primer pSB1(A2/A3/AK3)
J37024 Cut X P Primer UNKNOWN
Cre Cut X P Primer UNKNOWN
J04500 (16P) Cut S P Primer pSB1(A2/A3/AK3)
B0015 (1I) Cut X E Primer UNKNOWN
J37023 Cut S E Primer UNKNOWN
J37019 Cut S P Primer pSB1(A2/A3/AK3)
J37025 Cut X P Primer J37024A