Utilization of VBNC Microorganisms

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Utilization of VBNC Microorganisms in the
Environment
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Construction of a Metagenomic Library
Soil microorganisms
Recombinant vectors then transformed into E. coli
Metagenomic library in E. coli
Large fragments of DNA (gt50 kb) obtained from
unculturable soil microorganisms are ligated
into BAC vectors.
ASM News July, 2000, 66, 7, pp 411-417. Marcia
S. Osburne, Trudy H. Grossman, Paul R. August,
and Ian A. MacNeil
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Detection of Biological Active Compound for
Metagenome Clones
A)
B)
Indirubin C16H10N2O2 M.W 262.27
A) E. coli colonies carrying metagenome DNA
encoding family of pigments and
antibacterial small molecules. B) Structure of
the antileukemic drug indirubin.