Attachment and Contraction

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Attachment and Contraction
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FN Bead Binding Movement and Release
Displacement
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How does a cell treat the ECM?
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Unstressed Attachments
Choquet et al., Cell. 8839 (1997)
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Force Causes Reinforcement
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Force Causes GFP-Paxillin Accumulation in
RPTPa/ with FN
Without Force
FN Bead
Con A Bead
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RPTPa Deletion Blocks But Transfection Restores
Binding
-/- Cells with Force
-/- Transfected wtRPTPa
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Substrate Rigidity Can Control Direction of
Motility
Non-Rigid
Rigid
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Horwitz, Scientific American
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Summary of Course Lectures
13-Cytoskeleton 14-Self-Assembly 15-16Molec.Mech.Q
.doc 16-Molecular Motors 17-Mechanics 18-Motor
Kinetics 19 Protein Translocation 20 Fission and
Fusion 21 Proteol-Degrad 22 Signal Integration 23
Cell Migration 24 Cell Motility ()
1-Intro2-Diffusion3-Diff.Transport4-Nuclear
Organization5.Nuc.Binding6 DNA Replication7
RNA Transcription8-RNA FactorBind.doc9-Fluid
Bilayer10-Guoy-Chapman11-Membrane Potential12
Protein Translation