Title: Intersubject Normalization for Group Analyses in fMRI
1Intersubject Normalizationfor Group Analyses in
fMRI
Jody Culham Brain and Mind Institute Department
of Psychology University of Western Ontario
http//www.fmri4newbies.com/
Last Update January 18, 2012 Last Course
Psychology 9223, W2010, University of Western
Ontario
2Brains are Heterogeneous
Slide from Duke course
3How can we define regions?
- Talairach coordinates
- Example The FFA is at x 40, y -55, z -10
- Anatomical localization
- Example The FFA is in the right fusiform gyrus
at the level of the occipitotemporal junction
- Functional localization
- Example The FFA includes all voxels around the
fusiform gyrus that are activated by the
comparison between faces and objects
Kanwisher, McDermott Chun, 1997, J Neurosci
4Talairach Coordinate System
- Talairach Tournoux, 1988
- made an atlas based on one brain
- any brain can be squished or stretched to fit
hers and locations can be described using a 3D
coordinate system (x, y, z)
from an alcoholic old lady
Note Thats TalAIRach, not TAILarach!
5Rotate brain into ACPC plane
Find anterior commisure (AC)
Find posterior commisure (PC)
ACPC line horizontal axis
Note official Tal says to use top of AC and
bottom of PC but I suspect few people actually do
this
Source Duvernoy, 1999
6Deform brain into Talairach space
- Mark 8 points in the brain
- anterior commisure
- posterior commisure
- front
- back
- top
- bottom (of temporal lobe)
- left
- right
Extract 3 coordinates
7Talairach Daemon
8Talairach Tables
Source Culham et al., 2003, Exp. Brain Res.
- Talairach coordinates can be useful for other
people to check whether their activation foci are
similar to yours - Often its easiest to just put coordinates in a
table to avoid cluttering text
9Do We need a Tarailach Atras?
10MNI Space
- Researchers at the Montreal Neurological
Institute created a better template based on a
morphed average of hundreds of brains (not just
one brain like Talairach) - The MNI brain is more representative of average
brain shape however, it does not provide
Brodmann areas - The MNI alignment is more complex than Talairach
SPM uses it but many software packages still use
Talairach - CAVEAT The MNI and Talairach coordinate are
similar but not identical -- careful comparison
requires a transformation - -- converters can be found online
http//brainmap.org/icbm2tal/index.html
Source http//www.mrc-cbu.cam.ac.uk/personal/matt
hew.brett/abstracts/MNITal/mniposter.pdf
11Another Look at RFX
Huettel, Song McCarthy, 2008
12Smoothing and Averaging
Large activations across multiple subjects are
more likely to show common activation than small
ones --gt Need to smooth (esp. for RFX analyses)
UNDER CONSTRUCTION Need to make a slide that
shows this with real data
13Talairach Pros and Cons
- Advantages
- widespread system
- allows averaging of fMRI data between subjects
- allows researchers to compare activation foci
- relatively easy to use
- Disadvantages
- not appropriate for all brains (e.g., Japanese
brains dont fit well) - activation foci can vary considerably other
landmarks like sulci may be more reliable
14Left is what?!!!
Note Make sure you know what your magnet and
software are doing before publishing left/right
info!
Note If youre really unsure which side is
which, tape a vitamin E capsule to the one side
of the subjects head. It will show up on the
anatomical image. (Remember which side you put
it on!)
15Brodmanns Areas
Brodmann (1905) Based on cytoarchitectonics
study of differences in cortical layers between
areas Most common delineation of cortical
areas More recent schemes subdivide Brodmanns
areas into many smaller regions Monkey and human
Brodmanns areas not necessarily homologous
16Anatomical LocalizationSulci and Gyri
gray/white border
pial surface
SULCUS
FISSURE
GYRUS
Source Ludwig Klingler, 1956, in Tamraz
Comair, 2000
17Variability of Sulci
Source Szikla et al., 1977, in Tamraz Comair,
2000
18Variability of Functional Areas
- Watson et al., 1995
- - motion-selective area, MT (V5) is quite
variable in stereotaxic space - however, the area is quite consistent in its
location relative to sulci - junction of inferior temporal sulcus and lateral
occipital sulcus - see also Dumoulin et al., 2000
19Cortical Surfaces
segment gray-white matter boundary
inflate cortical surface
render cortical surface
sulci concave dark gray gyri convex light
gray
- Advantages
- surfaces are topologically more accurate
- alignment across sessions and experiments allows
task comparisons
20Cortical Inflation Movie
Movie unfoldorig.mpeg http//cogsci.ucsd.edu/ser
eno/unfoldorig.mpg Source Marty Serenos web
page
21Cortical Flattening
2) make cuts along the medial surface (Note,
one cut typically goes along the fundus of the
calcarine sulcus though in this example the cut
was placed below)
1) inflate the brain
3) unfold the medial surface so the cortical
surface lies flat
4) correct for the distortions so that the true
cortical distances are preseved
Source Brain Voyager Getting Started Guide
22Spherical Averaging
Future directions of fMRI Use cortical surface
mapping coordinates Inflate the brain into a
sphere Use sulci and/or functional areas to match
subjects data to template Cite latitude
longitude of spherical coordinates
Movie brain2ellipse.mpeg http//cogsci.ucsd.edu/
sereno/coord1.mpg Source Marty Serenos web page
Source Fischl et al., 1999
23Spherical Averaging
Source MIT HST583 online course notes
24Learning Brain Anatomy
- Duvernoy, 1999, The Human Brain Surface, Blood
Supply, and Three-Dimensional Sectional Anatomy - beautiful pictures
- good schematic diagrams
- clear anatomy
- slices of real brain
- Springer, US439
- Ono, 1990, Atlas of the Cerebral Sulci
- great for showing intersubject variability
- gives probabilities of configurations and stats
on sulci - Theime, US199
- Damasio,2005, Human Brain Anatomy in Computerized
Images, 2nd edition - good for showing sulci across wide range of
slice planes - 2nd edition much better than 1st edition
- Oxford University Press, US100
- Tamraz Comair, 2000, Atlas of Regional Anatomy
of the Brain Using MRI with Functional
Correlations - good overview
Wanna get rich? Publish a brain atlas. Sheesh,
these are expensive!