Laser Microdissection (LMD)

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Laser Microdissection (LMD)
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Instructors

• Chicago Medical School (RFUMS)
• Christine Sanders geneskr_at_alumni.ucsd.edu
• Northern Louisiana Crime Lab (NLCL)
• Pat Wojtkiewicz pwojtkie_at_nlcl.org
• Kelli Raley kraley_at_nlcl.org
• Leica Microsystems
• Andy Lee Alee_at_leica-microsystems.com

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Student Introductions
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Facilities

• Where are we?
• Largo, FL
• NFSTC - National Forensic Science Technology
  Center
• http//nfstc.org/aboutus/index.htm
• Walking tour

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Agenda Review

• DAY 1
• Introduction to LMD technology
• Basic cell identification
• Preparing samples for LMD (lab)
• Demonstration and instruction of LMD
  instrumentation (lab)
• LMD exercises (lab)
• (NFSTC staff performs PCR and CE)

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Agenda Review cont

• DAY 2
• Research studies in forensic LMD development
• LMD applications using fluorescence (lab)
• LMD experiences in the forensic laboratory
• Review of DNA results from DAY 1 laboratory
  exercises
• Practical application of LMD in the forensic
  laboratory
• TL/Managers only - Round table discussions