Grind tissue in liquid N2

1
7 ml Trizol/g tissue (use glass pipettes)
Grind tissue in liquid N2
Thaw on ice
vortex
Shake for 5m _at_ RT
Hand shake for 15s _at_ RT
0.7ml CHCl3/ml Trizol
Spin _at_ 3500 krpm X 15m
Aqueous phase to 50 ml tubes
Measure approximate volume
organic phase
Spin _at_ 3500 krpm X 15m
Rock X 10m_at_ RTSP
0.7vol 2-propanol