DataCentric Automation (DCA)

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DataCentric Automation (DCA)

• A subsidiary of Titan Corporation
• Based in Nashville, TN
• Extensive lab automation experience
• Software development
• Database design
• Hardware integration
• Customers and Partners
• Syrrx
• Novartis Institute
• GlaxoSmithKline
• Northeast Structural Genomics Consortium

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The Rhombix Series

• Automated solutions for protein crystallization
• Integrated Solution Rhombix OPUS
• Automated Systems Rhombix Screen
• Rhombix Vision
• Workstations Rhombix Screen LT
• Rhombix Vision LT

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The Rhombix OPUS
Integrated Solution For Protein Crystallization
R h o m b i x O P U S
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The Rhombix Series
Fully-Automated Systems
Prepare Reagents
Assemble Crystal Plates
Seal Crystal Plates
Incubate Crystal Plates
Image Crystal Plates
Score Crystal Results
Rhombix Screen Automated Liquid Handling
Rhombix Vision Automated Plate Imaging
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The Rhombix Series
Semi-Automated Workstations
Prepare Reagents
Assemble Crystal Plates
Image Crystal Plates
Score Crystal Results
Rhombix Screen LT Liquid Handling Workstation
Rhombix Vision LT Imaging Workstation
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Rhombix Features

• Automation of all aspects of crystallization
• Modular system architecture
• Modular hardware components and software tools
• Add components and scale automation as throughput
  requirements increase
• Upgrade individual components to reduce cost of
  obsolescence
• Built on the Centrix Automation Engine

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Centrix Automation Engine
Prepare Reagent
Assemble Crystal Plate
Incubate Plate
Capture Image
Centrix Database
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Rhombix OPUS

• First commercial integrated protein
  crystallization system
• Automates all aspects of crystallization process
• Experimental design
• Reagent preparation
• Plate assembly
• Incubation
• Plate imaging crystal scoring
• Co-developed with GlaxoSmithKline

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Rhombix OPUS
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OPUS Process
Optimized Screens
Integrated Crystallization Process
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Building Crystallization Plate

• Best-of-Breed liquid handlers
• Hamilton MicroLab STAR
• Cartesian Honeybee
• Low-volume, non-contact
• Comprehensive experimental design software
• Reagent design
• Plate mapping

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Reagent Prep/Plate Assembly Hardware
Mitsubishi Robotic Arm
Hamilton MicroLab STAR
Cartesian Honeybee Dispenser
KBiosystems Pressure Plate Sealer
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Deck Layout for Building Reagents
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Defining Chemical Reagents
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Defining Screens (1 of 3)
User lays out grids and defines reagent
distributions across wells. Note in this view
that distributions appear graphically.
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Defining Screens (2 of 3)
In this view, the selected reagents distribution
appears numerically as well as graphically.
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Defining Screens (3 of 3)
User can choose from many reagent distribution
patterns
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Defining Protein Reagents
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Defining Experiments
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Plate Assembly Process
1a. Transfer of Protein To Shelf (gt1 uL)
1b. Transfer of Protein To Shelf (lt1 uL)
3. Seal Plate
2b. Transfer of Reagent to Shelf (lt 1 uL)
2a. Transfer of Reagent to Shelf (gt 1 uL)
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Plate Incubation and Imaging

• Integrated Plate Storage and Imaging
• No change in plate temperature
• True color CCD camera
• First multi-mode plate imager
• Dark-field, Bright-field, Polarized
• Data fusion
• Illumination types
• Focus depths
• Flexible scheduling software
• Any plate, any time

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Plate Imaging and Incubation
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Plate Imaging and Storage
Random Access Plate Storage Hotel for SBS Plates
Environmental Enclosure
Linbro Plate Storage Matrix
Plate Imager
Robotic Arm For Plate Transfer
Manual Access Plate Hotel
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Plate Imaging Unit
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Imaging of 96-well Sitting Drop Plate
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Clarix Software
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Monitoring Experiments
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Clarix Software

• Image analysis and annotation
• Flexible viewing options
• Full image
• Thumbnails of plate
• Time progression
• Multiple lighting options
• Multiple export formats
• Powerful query tools for data mining

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The Rhombix Series Modular Architecture
R h o m b i x O P U S
Design Experiment
Prepare Reagents
Assemble Crystal Plates
Seal Crystal Plates
Incubate Crystal Plates
Image Crystal Plates
Score Crystal Results
Rhombix Screen Automated Liquid Handling
Rhombix Vision Automated Plate Imaging
Rhombix Screen LT Liquid Handling Workstation
Rhombix Vision LT Imaging Workstation
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