Nutritional Requirements of Tissue culture

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Nutritional Requirements of Tissue culture

1. Salts. 2. Carbohydrate . 3. Amino acids. 4.
   Vitamins.

Basic Principles of Tissue culture

• Environment ( Temperature,hydrogen ion
• concentration , essential metabolites , matrix
  ).
• 2. Prevention of contamination, Removal of
  harmful
• Metabolites.

• Applications
• Embryoloy ( IVF, Stem cells Cloning).
• Virology ( Vaccines).
• Genetic ( Human genome , karyotype of diseases,
  etc.

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Tissue culture

• Kinds of techniques
• Culture in clot of cover slip.
• Culture on a watch glass .
• Culture on petri dishes.
• Culture on ordinary test tubes , Erlenmeyer flask
  , round bottomed flasks.

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Steps of IVF

• In Vitro Fertilization (IVF) provides an
  alternative method of achieving a pregnancy for
  couples who have tried and have been unsuccessful
  with other fertility treatment.
• steps are as follows
• Retrieval of the mature egg.
• Sperm collection, fertilization, and embryo
  development. 
• Transfer of the developing embryo(s) into the
  uterus.
• Implantation and growth of the embryo(s). 

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•  
• Information for all prospective IVF patients
• All female partners entering the program be
  tested for antibodies against Rubella. 
• Alcohol, and cigarette smoking should be
  eliminated during the IVF cycle. 
• Sitting in hot tubs and saunas is not recommended
  during the IVF cycle.
• Do not begin any new exercise, sport, or intense
  athletic training during the IVF cycle.
• All women attempting conception should be taking
  a specific prenatal vitamin.  No other
  supplements should be taken unless specifically
  prescribed.

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IVF
Application of tissue Culture
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Application of tissue Culture
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