URINALYSIS AND BODY FLUIDS (SEMINAL FLUID) LECTURE - PowerPoint PPT Presentation

About This Presentation
Title:

URINALYSIS AND BODY FLUIDS (SEMINAL FLUID) LECTURE

Description:

URINALYSIS AND BODY FLUIDS (SEMINAL FLUID) LECTURE Dr. Essam H. Jiffri SPERM COUNT - Fertilization is accomplished by one spermatozoa. - Normal values are commonly ... – PowerPoint PPT presentation

Number of Views:340
Avg rating:3.0/5.0
Slides: 20
Provided by: Adm9108
Category:

less

Transcript and Presenter's Notes

Title: URINALYSIS AND BODY FLUIDS (SEMINAL FLUID) LECTURE


1
URINALYSIS AND BODY FLUIDS (SEMINAL
FLUID)LECTURE
  • Dr. Essam H. Jiffri

2
SPERM COUNT
  • - Fertilization is accomplished by one
    spermatozoa.
  • - Normal values are commonly listed as 20 to160
    million sperm per milliliter, with counts between
    10 and 20 million per milliliter considered
    borderline.

3
SPERM COUNT
  • - The sperm count is performed in the same manner
    as blood and CSF counts that is by diluting the
    specimen and counting the cells in a Neubauer
    chamber.
  • - The amount of the dilution and the number of
    squares counted vary among laboratories.

4
Two frequently used methods dilute the specimen
120 and count the sperm in either the five RBC
squares or in two large WBC squares.
5
SPERM COUNT
  • - When the five RBC squares are used, the number
    of sperm counted is multiplied by
  • 1,000,000 to calculate the number of sperm per
    milliliter.
  • - If the two WBC squares are used, the count is
    multiplied by 100,000 to achieve the same result.

6
(No Transcript)
7
SPERM COUNT
  • - The traditional diluting fluid contains sodium
    bicarbonate and formalin, which immobilize and
    preserve the cells, good results can also be
    achieved using tap water.

8
Motility
  • - Laboratory evaluation of sperm motility is
    performed by examining the undiluted specimen
    microscopically and determining the percentage of
    sperm showing active motility.

9
Motility
  • - Approximately 25 high-power field should be
    examined.
  • - A minimum motility of 50-60 percent with a
    quality of fair (2.0) is considered normal for
    specimens tested within the 3-hour time period.

10
Morphology
  • - The presence of a normal number of sperm that
    are non-motile will produce infertility, and the
    presence of sperm that are morphologically
    incapable of fertilization also will result in
    infertility.

11
Morphology
  • - Sperm morphology is evaluated with respect to
    both head and tail appearance.
  • - The normal sperm has an oval head measuring
    approximately 3 x 5 µm and a long tapring tail.

12
Morphology
  • - Abnormality in head structures are associated
    with poor ovum penetration and include double
    heads, giant and morphous heads, pin heads,
    tapering heads, and constricted heads.

13
(No Transcript)
14
Morphology
  • - Motility is impeded in sperm with double or
    coiled tails.
  • - Immature sperm (spermatids) also may be present
    and must be differentiated from white blood
    cells.
  • - They are more spherical than mature sperm and
    may or may not have tails.

15
Morphology
  • - Sperm morphology should be reported from a
    stained specimen examined under oil immersion,
    the recommended stain is the Papanicolaou, and if
    this is not available acceptable results can be
    obtained by using hematoxylin, crystal violet, or
    Giemsa stains.

16
Morphology
  • - A specimen that contains less than 30 percent
    abnormal forms is considered normal.
  • - Additional tests may be requested, the most
    common are tests for sperm viability, seminal
    fluid fructose level, and sperm agglutinins.

17
Morphology
  • - Viability is tested by mixing the specimen with
    an eosin-nigrosin stain and examining
    microscopically for dead cells that stain red
    against a purple background.
  • - Living cells are not infiltrated by the eosin
    dye and remain a bluish-white color.

18
Morphology
  • - It is not common to find viable sperm in a
    postvasectomy patient, and care should be taken
    to overlook even a single sperm.
  • - Specimens are routinely tested at monthly
    intervals beginning at 2 months postvasectomy and
    continuing until 2 consecutive monthly specimens
    show no spermatozoa.

19
Normal values for Semen Analysis
  • ________________________________________
  • Volume 2-5 ml
  • Viscosity Pours in droplets
  • pH 7.3-7.8
  • Count 20-160 million/ml
  • Motility gt 50-60 within 3 hours
  • Quality gt 2.0 of fair
  • Morphology lt 30 Abnormal forms
  • ________________________________________
Write a Comment
User Comments (0)
About PowerShow.com