Halosperm

1
Halosperm Training slides
2
How to calculate the DNA Fragmentation Index
(DFI)

• After sample processing with Halosperm, stain
  the slide with DIFF-QUICK/Panoptic stain
• Check the sample with a bright field microscope,
  and classify 300-500 sperm cells as follows

3
Training slides. How to use them

• Use the first 5 slides to learn how to classify
  each sperm cell.
• Use the last 3 slides to practice calculating the
  DFI on your own.

4
fragmented
normal
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fragmented
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fragmented
fragmented
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Normal 10 Fragmented 4 DFI 28.5
normal
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5
normal
fragmented
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fragmented
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fragmented
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Normal 16 Fragmented 7 DFI 30.4
fragmented
normal
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fragmented
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fragmented
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fragmented
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6
normal
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fragmented
normal
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Normal 19 Fragmented 1 DFI 5
normal
7
normal
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fragmented
normal
Normal 15 Fragmented 3 DFI 16.6
fragmented
normal
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fragmented
8
normal
normal
normal
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fragmented
fragmented
normal
normal
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normal
fragmented
Normal 10 Fragmented 3 DFI 23
normal
normal
9
Normal 16 Fragmented 7 DFI30.4
10
Normal 21 Fragmented 6 Degraded1 DFI 25
11
Normal 10 Fragmented 3 DFI 23