Interaction of nanostructured silicon with the living matter

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Interaction of nanostructured silicon with the
living matter

• Irina Kleps
• National Institute for Research in
  Microtechnologies, (IMT-Bucharest), P.O.Box
  38-160, Bucharest, Romania, fax [phone],
  tel [phone] e-mail irinak_at_imt.ro

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MAIN PARTNERS

• Institute of Microtechnics, Mainz, Germany
• NMRC, Cork, Ireland
• University of Bucharest, Faculty of Chemistry
• Institute of Biochemistry, Bucharest
• University of Bucharest, Faculty of Biology
  Institute Chemico-Pharmaceutical, Bucharest
• DEXTERCOM.SRL, Bucharest

Our team Anca Angelescu, Mihaela Miu, Monica
Simion, Florea Craciunoiu, Teodora Ignat, Adina
Bragaru and Irina Kleps
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Main results obtained in IMT-Bucharest
Laboratory of Nanotechnology
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Nanoelectrode array - SEM details
Some advantages of these electrodes (i) this
kind of nanoelectrodes can be fabricated through
the common photolithographic technique -
ultraviolet light proximity and does not involve
other special lithographic techniques (ii)
these electrodes are never recessed (iii) the
array consists of a series of nanoelectrodes
separated by an insulating material with
sufficient spacing to avoid the overlapping of
the diffusion layers.
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NE Biomedical applications eukaryote and
prokaryote cells
ELECTROCHEMICAL CELL ON A CHIP
CHO cells on NE array

• investigation of the electrochemical bacteria
  activity
• study of the electron transfer from living
  cells to the electrode arrays by electrochemical
  measurements
• conduction measurement on living cell cultures

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Nanostructured silicon reactor for cell
investigation
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POROUS SILICON presents the advantage over other
bioactive materials because it is compatible with
silicon integrated circuit technology
The lab-on-a-chip with POROUS SILICON reactor
integrated with other electrical and optical Si
devices is an useful tool to investigate cells
answer to various stimuli such as drug solutions,
electric impulses, or change of the temperature
PS surface functionalisation can be achieved by
thermal, chemical and physico-chemical
treatments (i) thermal treatments in O2 (two
steps process) (ii) thin carbon, or silicon
carbide layer deposition (iii) surface
derivatization
POROUS SILICON is a biocompatible and bioactive
substrate which facilitates the adhesion and
proliferation of various cell
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CHO cells on 31 PS / Sip (100), with the
surface modified by a 30nm carbon (10x)
PS/pSi (100), 31 porosity, annealed in N2 and
cultivated with B16-F1 cells (20x)
Fluorescence images obtained for CHO cell lines
grown on PS surface after 1 and 3 days
respectively
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Resorbable porous silicon nanocomposite
reservoirs for mineral or drug delivery
Fe(NO3)3 deposition as powder, followed by
melting at 470C
Spin-on of a mixing etoxyetanol and Fe(NO3)3
Samples with Fe / PS / Si n (cross section)

• Fe release was investigated in SBF solution.
• In the first days a strong Fe release was
  observed due to the dissolution of the iron salt
  from the PS pores
• After that the iron release takes place in the
  same time with the degradation of the PS matrix
  in SBF solution.

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DNA microchip
A silicon microchips with a microreactor and a
heater for oligonucleotide amplification by
polymerase chain reaction - PCR and rapid
analysis of the DNA samples
Functionalized reservoir with poly
(lysine).Fluorescent primer immobilisation, 10
pmols / µl
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Microfluidic Biochip for Biomedical Application
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Recent published papers

• I. Kleps, A. Angelescu, M. Miu, M. Simion, A.
  Bragaru, M. Avram, Nanoelectrodes on silicon for
  electrochemical applications, in NATO ASI
  Synthesis, Functional Properties and Applications
  of Nanostructures, (2002)
• I. Kleps, A. Angelescu, M. Miu, Materials Science
  Engineering C-Biomimetic and Supramolecular
  Systems 19219-223 (2002).
• Irina Kleps, Anca Angelescu, Marioara Avram,
  Mihaela Miu, Monica Simion, Microelectronic
  Engineering 61-62 (2002) 675-680.
• Irina Kleps, "Electrochemical nanoelectrodes", in
  "Encyclopedia of Nanoscience and Nanotechnology",
  H. S. Nalwa, (Ed.), American Scientific
  Publishers, 793-817, 2004.
• A. Angelescu, I. Kleps, M. Miu, L. Zdrentu, S.
  Petrescu, M. Simion, A. Bragaru, T.
  Neghina,Nanostructured silicon from material to
  devices for biomedical applications
  Nanoscience and Nanotechnology 4, Heron Press,
  Sofia, 2004, pp 280-283
• M. Miu, I. Kleps, A. Angelescu, T. Ignat, A.
  Bragaru, M. Simion, S. Petrescu, L. Zdrentu, N.
  Moldovan, M. Modreanu, P. Rosengrave, Porous
  silicon a nanostructured biomaterial,
  Nanoscience and Nanoengineering Vol., Series in
  Micro and Nanoengineering, Romanian Academy
  Editure, Bucuresti 2004.
• A. Angelescu, I. Kleps, M. Miu, S. Petrescu, C.
  Paduraru, M. Simion, A. Bragaru, A.
  Raducanu,Thin carbon layers on nanostructured
  silicon -properties and applications, Frontiers
  of Multifunctional Integrated Nanosystems,
  Kluwer Academic Publishers, 2004, 197-204.
• M. Miu, A. Angelescu, I. Kleps, M. Simion, A.
  Bragaru, T. Neghina, M. Modreanu, D. Iacopino,
  P. Roseingrave,Porous silicon matrix as mineral
  microreservoir, CAS 2003 Proceedings, vol. 1,
  26th edition, oct. 8-12, 2003, p. 99-105
• A. Angelescu, I. Kleps, M. Miu, T. Neghina, M.
  Simion, A. Bragaru, M. Giurginca, Metal-porous
  silicon composite layers for biomedical
  applications, European Micro and Nanosystems
  2004 (EMN04), Paris, Franta, 20-21 octombrie,
  2004
• Monica Simion, Oana Tatiana Nedelcu, Irina
  Kleps, Anca Angelescu, Mihaela Miu,Electro -
  Thermal Simulation For PCR Silicon Chips,
  Spring College on Science at Nanoscale , Trieste
  Italia