Fingerstick Blood Collection into a Microtainer for PNG

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Fingerstick Blood Collection into a Microtainer
for PNG
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Universal precautions

• Assume that all human blood is potentially
  infectious for HIV, hepatitis, and other
  infectious agents.
• Practice universal precautions using gloves, eye
  protection, and lab coats.
• Always use sterile, single-use, disposable
  supplies for sample collection.

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Finger stick procedure

1. Place all collection materials on top of
   disposable pad. Open the alcohol swabs, gauze,
   and bandage. Have all items ready for blood
   collection. Attach the patients labels to the
   malaria slide and DBS card.

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Finger stick procedure

1. Put on your powder free gloves. Turn
   participants hand upward. Massage participants
   hand and lower part of the finger to increase
   blood flow.

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Finger stick procedure

1. Scrub the participants middle finger or ring
   finger with an alcohol swab. Dry with gauze.

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Finger stick procedure

1. Hold the finger in an upward position and lance
   the palm side surface of the finger (between the
   nail and the finger pad) with the lancet. Press
   firmly on the finger when making the puncture.
   Doing so will help you to obtain the amount of
   blood you need.

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Finger stick procedure

1. Apply slight pressure to start blood flow. Blot
   the first drop of blood on a gauze pad and
   discard in appropriate container.

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Finger stick procedure

1. Keep the finger in a downward position and gently
   massage it to maintain blood flow. Hold the
   Microtainer at an angle of 30 degrees below the
   collection site and use the scoop on the
   Microtainer to guide the drop into the vial. Do
   not scrape the skin. Fill the Microtainer to
   350 - 500 µL level.

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Finger stick procedure

1. Cap the Microtainer and gently invert it 10
   times to prevent clots from forming.

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Finger stick procedure

1. Apply a sterile adhesive bandage over the
   puncture site.

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Finger stick procedure

1. Place the label on the vial. If the label
   contains a barcode, the barcode needs to be
   vertical like a ladder when placed on the vial.
   If the barcode is not vertical, the laboratory
   will not be able to read the label. Place the
   label from left to right starting from the cap
   end and leave the graduated numbers on the tube
   visible.
2. Properly discard all used materials.

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Any questions?