Title: Principles of measurement
1(No Transcript)
2 DC Detection Method
The Pulse
Dilutions for Semiautomatic Analyser
- First Dilution
- 1500 (WBC)
- Second Dilution
- 150.000 (RBC)
-
Sample Beaker DB - 1
3 DC Detection Method
The Pulse
Dilutions for Semiautomatic Analyser
- WBC Dilution
- 3 Drops Quicklyser for
- Lysing and HGB
RBC Dilution
4 DC Detection Method
The Pulse
External Electrode
Aperture
Internal Electrode
5 DC Detection Method
Transducer
Vacuum
External Electrode
Blood cells
6 DC Detection Method
Transducer
External Electrode
Aperture
Internal Electrode
7 DC Detection Method
The Pulse
External Electrode
Aperture
Internal Electrode
U R x I
8 DC Detection Method
The Pulse
External Electrode
Aperture
Internal Electrode
U R x I
9 DC Detection Method
Absolute Counting I
Time sec
Counting Time
forerun
afterrun
(defined Volume)
Transducer
Stop-Sensor
Start-Sensor
Manometer
Blood suspension
10 DC Detection Method
Absolute Counting I
Time sec
Counting Time
Prerun
Afterrun
(defined Volume)
Transducer
Stop-Sensor
Start-Sensor
Manometer
Blood suspension
11 DC Detection Method
Absolute Counting I
Time sec
Counting Time
Prerun
Afterrun
(defined Volume)
Transducer
Stop-Sensor
Start-Sensor
Manometer
Blood suspension
12 DC Detection Method
Absolute Counting I
Time sec
Counting Time
Prerun
Afterrun
(defined Volume)
Transducer
Stop-Sensor
Start-Sensor
Manometer
Blood suspension
13 DC Detection Method
Absolute Counting I
Time sec
Counting Time
Prerun
Afterrrun
(defined Volume)
Transducer
Stop-Sensor
Start-Sensor
Manometer
Blood suspension
14 DC Detection Method
Absolute Counting
15 DC Detection Method
Absolute Counting II
- Not to be changed by UserSample VolumeDilution
RatioCounted VolumeAperture Diameter - Automatically checked by the instrumentCounting
TimeCondition of aperture during
measurement(Noise-Control) - AdvantagesNo calibration of countersAutomatic
check of aperture
16 DC Detection Method
Relative Counting
17 DC Detection Method
Comparison of Absolute and Relative Counting
Absolute Counting
Relative Counting
- Measurement of particlesin a defined volume
- Calculation of cell-concentration with known
Sample volume and defined Sample dilution - no calibration of counting values
- Measurement of particles per time period
- The count is received indirectly through a
reference-sample. It is taken for granted that
the sample and the reference-sample have the same
characteristics and give the same result. - Calibration of counting values in necessary
18 DC Detection Method
Volume (Time)
Pulse Height
19 DC Detection Method
Histogram
Pulse Picture
1
2
3
4
5
6
7
8
9
10
11
12
13
14
Cumulative Size Distribution Curve
1
2
3
4
5
6
7
8
9
10
11
12
13
14
30
20
10
4
1
0
0
0
1
2
3
4
5
4
3
2
1
20 DC Detection Method
Histogram
Cumulative Size Distribution Curve
1
2
3
4
5
6
7
8
9
10
11
12
13
14
30
20
10
4
1
0
0
0
1
2
3
4
5
4
3
2
1
Histogram
1
2
3
4
5
6
7
8
9
10
11
12
13
14
4
1
0
0
0
1
2
3
4
5
4
3
2
1
21 DC Detection Method
Histogram
Erythrocyte Histogram
25-75 fl
200-250 fl
22 DC Detection Method
Histogram
Platelet Histogram
fixed at 12 fl
2-6 fl
12-30 fl
23 DC Detection Method
Histogram
24 Operation Sequences
Histogram
READY-Status
- All solenoid valves are de-energized.
- C (Common) and NO (Normally Open)
- solenoid valve ports are connected.
- Ball Float is in the down position.
25 Operation Sequences
Histogram
COUNT KEY on
- SV1 and SV 4 are energized.
- C and NC solenoid valve ports are
- connected.
- Diluent is aspirated through
- SV4, SV2, Transducer, SV1 and SV3.
- The Ball Float in the manometer is
- pushed upward.
- The inner portion of transducer and
- manometer is automatically rinsed to
- minimize carry-over effect.
26 Operation Sequences
Histogram
Counting
- SV1 and SV4 are de-energized and
- SV2 is energized.
- The diluted sample is aspirated through
- the aperture and the Ball Float moves
- downward.
- When the Ball Float reaches level A,
- the counter starts counting.
- When the Ball Float reaches level B,
- the counting stops and SV2 is
- de-energized.
- The volume of aspirated sample is the
- same as the manometer-volume
- between points A and B.
27 Operation Sequences
Histogram
Clog removal
- SV1 and SV3 are energized.
- Pressure Pump is activated.
- Pressure is applied to the aperture to
- remove the clog.
- The same function occurs if the
- FLUSH key is pressed.
28 Operation Sequences
Histogram
Diluent Fill
- When power switch is turned on,
- SV1 and SV4 are energized.
- Diluent is aspirated through the
- transducer.
- The same function occurs if the
- FILL key is pressed.
- The diluent removes diluent and any
- blood sample remaining in the system.
29Haemoglobin
Histogram
- Haemoglobin is the dye at the RBC.
- It is a Fe-containing Protein.
- Haemoglobin is exclusively produced by
Erythroblasts in the bone marrow. - Hemoglobin is an important part of the
Erythrocytes. - There is a direct relation between Haematokrit,
Erythrocyte-concentration and Haemoglobin. - The main function of Haemoglobin in the body is
to transport Oxygen and CO2.
30Haemoglobin
Histogram
Patient Limits
31Haemoglobin
Histogram
Sysmex KCN-Method
- A special reagent is needed, containing
Cyanidefor lysing RBC - F-Series Quicklyser
32Haemoglobin
Histogram
Sysmex SLS-Method
- SLS means Sodiumlaurylsulfate
- SLS ins not toxic (ingredient in a lot of soaps
tooth-paste) - Sysmex Sulfolyser is now used with the majority
of instruments (K-, E-, NE-,SF-,SE-, XE-systems) - Only Sysmex hematology systems have a stable
KCN-free Haemoglobin-measurement as standard.
33Haemoglobin
Haemoglobin- Measurement
Heamoglobin molecule
Hydrophobic area
-
SO
3
Na
Hydrophilic area
34Haemoglobin
Cyanide-free SLS-method
Haemoglobin molecule
a
a
Hydrophobic area
a
-
SO
3
Na
b
b
Hydrophilic area
35Haemoglobin
Cyanide-free SLS-method
- Hydrophobic area of SLS bind to Globin
- Conformation change
- Oxidation Fe2 --gt Fe3
- Hydrophilic area of SLS bind to Fe3 --gt SLS-Hb.
Fe 3
Fe 3
Fe 2
SLS
36Haemoglobin
Cyanide-free SLS-method
- photometric measurement at 540 nm (F-Series,
K-800, K-1000, K-4500)
Sample
Cellpack
Photosensor
Lamp
Flowcell
Lens Filter
37Haemoglobin
First step BLANK MEASUREMANT
Lamp
38Haemoglobin
Second step MEASUREMENT WITH LYSED SAMPLE
Lamp
39Haemoglobin
Start
Flow Cell is rinsed with Diluent.
Measurement and saving of Blank.
Measurement of sample dilution
Sample value -Blank HB-value
Printing of result
Lamp
End
40Haematocrit
Haematocrit
- Haematocrit is determined by comparing the total
or cumulative volume of red blood cells to the
volume of whole blood. - Determined normally by centrifugation or by
electric pulse measurement - Haematocrit is besides Haemoglobin an important
parameter in anemia-diagnosis.
41Haematocrit
Patient Limits
Newborn 0,51 - 0,65 Children 0,35 - 0,43 Women
0,38 - 0,48 Men 0,42 - 0,52
42Haematocrit
Centrifugation
- Determining the volume of corpuscles in the blood
- Normally equivalent to mass of erythrocytes.
- Blood is drawn into special capillaries, which
contain dried heparin. - After closing, capillaries are placed into a
centrifuge. - In the centrifuge blood is divided into its
corpuscular (cells) and fluid (plasma) components
43Haematocrit
Haematocrit- Measurement
44Haematocrit
Centrifugation
45Haematocrit
Centrifugation
Total volume VT
46Haematocrit
Centrifugation
Total volume VT
Centrifuge
47Haematocrit
Centrifugation
Total volume VT
Centrifuge
VT
Hct () V / VT x100
V
48Haematocrit
Centrifugation
Centrifugation
- Reading is done with a scale out of triangles.
- The auxiliary line through the borderbetween
erythrocytes- and leukocytesgives the
heamatocrit. - CV app. 1
49Haematocrit
Centrifugation
Pulse Height
Transducer
50Haematocrit
Centrifugation
Pulse Height
Transducer
Start-Sensor
defined volume VT
Stop-Sensor
51Haematocrit
Centrifugation
Pulse Height
Transducer
Start-Sensor
defined volume VT
Stop-Sensor
52Haematocrit
Centrifugation
Pulse Height
Transducer
Start-Sensor
defined volume VT
Stop-Sensor
53Haematocrit
Centrifugation
Pulse Height
Transducer
Start-Sensor
defined volume VT
Stop-Sensor
54Haematocrit
Centrifugation
Pulse Height
Transducer
Start-Sensor
defined volume VT
Stop-Sensor
55Haematocrit
Centrifugation
Pulse Height
Transducer
Start-Sensor
defined volume VT
Stop-Sensor
56Haematocrit
Centrifugation
Pulse Height
Transducer
Start-Sensor
defined volume VT
Stop-Sensor
57Haematocrit
Centrifugation
Pulse Height
Ph k x VEry VEry 1 / k x Ph Ph Pulse
hight k Constant VEry Volume of 1
Erythrocyte
Pulse Diagram
Ph
58Haematocrit
Centrifugation
Cumulative Pulse
Hct () V / VT x 100 V å VEry
VT
V
Ph Pulse hight k Constant VEry
Volume of 1 Erythrocyte
59Haematocrit
K-Series
Cumulative Pulse Height Detection
60Erythrocyte Indices Calculated (Parameters)
61Erythrocyte-Indices
RBC-Indices
62Biological Variation
Biological Variation
of quantitative parameters of CBC
Deviation from Median in
100
80
60
40
20
0
-20
-40
63Specifications
Specifications Example SYSMEX K-4500
64Specifications
- 18 ParameterWBC, RBC, HBG, HCT, PLTMCV, MCH,
MCHRDW-CV, RDW-SD, MPV, PDW, P-LCRSCR
(LYMPH), MCR (MONO/EO/BASO), LCR (NEUT)SCC
(LYMPH), MCC (MONO/EO/BASO), LCC (NEUT) - 3 HistogramsWBC, RBC, PLT
65Reliable Measurement Principles forhighest
accuracy
Specifications
WBC/RBC/PLT DC Detection Method HBG SLS-Haemogl
obin Method HCT Cumulative Pulse Height
Detection WBC-Diff DC Detection Method
66Specifications
- Throughput
- 80 Samples / Hour
- Cycle Time with single Samples
- 45 Seconds / Sample
67Specifications
- Sample Volume
- Closed Mode (Sampler) 200 µl
- Manual Open Mode 100 µl
- MCP-Mode 200 µl
- Capillary Mode gt 40 µ
68Parameters WBC, NEUT, LYMPH, EO/MONO/BASO,
NEUT, LYMPH, EO/MONO/BASO, RBC, HKT, HGB,
MCV, MCH, MCHC, RDW-SD, RDW-CV, PLT, PDW, MPV,
P-LCR Reagents Diluent CELLPACK Lyse-Reagent STR
OMATOLYSER-3WP haemoglobin-Reagent
SULFOLYSER Cleaning Reagent (Detergent) CELLCLEA
N Diameter of Apertures WBC 100
µm RBC/HCT/PLT 75 µm Throughput 80 Samples /
Hour (appr. 45 Seconds Cycle-Time /
Sample) Principles - DC Detection Method (RBC,
WBC, PLT) - SLS-Hemoglobin (HGB) - Cumulative
Pulse Height Detection - Calculation from RBC
and HCT (MCV) - Calculation from RBC and HGB
(MCH) - Calculation from HCT and HGB
(MCHC) Sample Volume Sampler Mode 200 µl Closed
Mode 200 µl Manual Open Mode 100
µl MCP-Mode 200 µl Capillary Blood Mode gt 40
µl Data Memory External data memory available
(K-DPS)
69Specifications
-Precision One fresh normal whole blood sample
was measured 10 times consecutively in Sampler
Mode. From those results the Coefficient of
Variation has been calculated. WBC CV lt
3,0 RBC CV lt 1,5 HGB CV lt 1,5 HCT CV lt
1,5 MCV CV lt 1,5 MCH CV lt 1,5 MCHC CV lt
1,5 PLT CV lt 4,0 Accuracy WBC 3,0 RBC
3,0 PLT 3,0 Linearity - WBC within
0,2 x 103/µl for 1,0 - 6,6 x 103/µl in Sampler
Mode or 3,0 für 6,7 - 99,9 x
103/µl RBC within 0,03 x 106/µl for 0,30 -
0,99 x 06/µl or 3,0 for 1,0 - 99,9 x
106/µl HGB within 0,1 g/dl for 0,1 - 10,0
g/dl or 1,0 for 10,1 - 20,0 g/dl and 2,0
for 20,1 - 25,0 g/dl HCT within 1,0 for
10,0 - 33,3 or 2,0 for 33,4 - 60,0
PLT within 10 X 103/µl for 10 - 199 x
103/µl or 5,0 for 200-999 x 103/µl (if
RBC lt 7,00 x 106/µl) Blank WBC lt 0,3 x
103/µl RBC lt 0,02 x 106/µl HBG lt 0,1
g/dl PLT lt 10 x 103/µl
70Display Range WBC 0,00-300,0 x103/µl RBC 0,00-20
,00 x 106/µl HGB 0,0-25,0 g/dl HCT 0,0-100
PLT 0-2000 x 103/µl Ports Serial port for
Host or K-DPS Parallel port for data printer
DP-510/490 Size Main Unit 580 x 600 x 31 Height
x Width x Depth Sampler 580 x 302 x
280 mm Power Supply 230 V AC/DC at 50
Hz Barcode-Reading CODABAR, CODE-11, CODE-39,
CODE-128, Interleaved 2 of 5, JAN-8, JAN-13
71Sample Flow
Sample Flow
72Sample Flow
Flow Chart
Cellpack
Mixing- Chamber 1500
Stromatolyser-3WP
Sample Rotor Valve (SRV)
Sulfolyser
73Sample Flow
Flow Chart
Cellpack
Mixing- Chamber 1500
Stromatolyser-3WP
Sample Rotor Valve (SRV)
Sulfolyser
74Sample Flow
Flow Chart
Cellpack
Mixing- Chamber 1500
Stromatolyser-3WP
Sample Rotor Valve (SRV)
Sulfolyser
75Sample Flow
Flow Chart
Cellpack
Mixing- Chamber 1500
Stromatolyser-3WP
Sample Rotor Valve (SRV)
Sulfolyser
76Sample Flow
Flow Chart
Cellpack
Mixing- Chamber 1500
Stromatolyser-3WP
Sample Rotor Valve (SRV)
Sulfolyser
77Sample Flow
Flow Chart
Cellpack
Mixing- Chamber 1500
1,0 ml
WBC Channel 1250
0,5 ml
Stromatolyser-3WP
6 µl
Sample Rotor Valve (SRV)
3 µl
1,0 ml
Hb Photometer 1500
Sulfolyser
0,5 ml
78Sample Rotor Valve
Sample Flow
Sample Rotor Valve
Lower Part (fixed)
Pipette
79Sample Rotor Valve
Sample Flow
Sample Rotor Valve
Upper Part (fixed)
Middle Part (moving)
Lower Part (fixed)
Pipette
80Sample Rotor Valve
Sample Flow
Sample Rotor Valve
Upper Part (fixed)
Middle Part (moving)
Lower Part (fixed)
Pipette
81Whole Blood Sampling
Sample Flow
Sample Rotor Valve
Vacuum by Diaphragm-Pump
0,1 ml Whole Blood
82Sample Separation
Sample Flow
Sample Rotor Valve
6 µl (Hb)
12µl (WBC)
4 µl (RBC)
83RBC-Dilution
Sample Flow
Sample Rotor Valve
Mixing-Chamber
4 µl (RBC)
Diluent-Diaphragm-Pump
84Sampling for 2nd RBC-Dilution
Sample Flow
Sample Rotor Valve
Mixing-Chamber
2nd Dilution Diaphragm-Pump
85Separation for 2nd RBC-Dilution
Sample Flow
Sample Rotor Valve
40 µl
862nd RBC-Dilution
Sample Flow
Sample Rotor Valve
RBC-Channel
Diluent- Diaphragm-Pump
87WBC- and HGB- Sample Dilution
Sample Flow
Sample Rotor Valve
HGB-Flow Cell
WBC-Channel
6 µl (HGB)
12µl (WBC)
HGB-Diaphragm-Pump
Diluent- Diaphragm-Pump
88Histograms
Interpretation
Histograms
89Histograms
Interpretation
Normal Sample
NO. 4 DATE 9/10/95 1511 MODE Whole
Blood WBC 5,8 x 103/µl RBC 4,84
x106/µl HGB 13,7 g/dl HCT 42,0 MCV 86,8 fl M
CH 28,3 pg MCHC 32,6 g/dl PLT 257 x103/µl
Parameters for CBC Leukocyte-Histogram Lymp
hocytes given in and absolute valueEo, Mono,
Baso given in and absolute valueNeutrophils
given in and absolute value Erythrocyte-Histogr
am Erythrocyte Distribution Width Platelet-Hist
ogramPlatelet Distribution WidthMean Platelet
VolumePlatelet - Large Cell Ratio
WBC
WBC
300
LYMPH 31,2 MXD 6,8 NEUT 62,0 LYMPH 1,8 x
103/µl MXD 0,4 x103/µl NEUT 3,6 x103/µl
RBC
RBC
250
RDW-SD 40,0 fl
PLT
PLT
40
PDW 13,1 fl MPV 10,4 fl P-LCR 28,1
90Histograms
RBC- and PLT-Histograms
- The two distribution curves are separated from
each other by a moving auto discriminator looking
to the Plateau. - Platelets have a size between 8 and 12 fl and are
counted between 2 and 30 fl. - Erythrocytes have a size of 80-100 fl and are
counted between 25 and 250 fl.
91Histograms
Erythrocytes
RBC- and PLT-Histograms
Base line
250 fl
2 fl
- The Size Distribution Curve should always start
on the base line and fall between the lower and
the upper discriminator.
92Histograms
Erythrocytes
Erythrocyte Histogram Flags
Mark RL , abnormal height at lower
discriminator
LD
RBC
The curve does not start at the base line.
PLT
- Possible causes
- Giant Platelets
- Micro-Erythrocytes
- Platelet Clumps
93Histograms
Erythrocytes
Erythrocyte Histogram Flags
Mark RU , abnormal height at the upper
discriminator.
UD
UD
RBC
RBC
The curve does not end at the base line.
- Possible causes
- Cold Agglutinins
- Erythroblasts / Normoblasts
94Histograms
Erythrocytes
Erythrocyte Histogram Flags
MP , multiple peaks found
RBC
RBC
95Histograms
Erythrocytes
Erythrocyte Histogram Flags
DW , abnormal histogram distribution
RBC
Distribution curve does not cross 20 level twice
96Histograms
Erythrocytes
Red Blood Cell Distribution Width
RDW-CV () 100 x s/µ
RDW-CV 11 - 16
68,26
97Histograms
Thrombocyte Histogram
2 Discriminators
30 fl
98Histograms
Thromboyte Histogram
- PLT, Count of platelets
- Histogram parameters
- MPV, mean platelet volumeReference range 8 - 12
fl - P-LCR, ratio of large plateletsReference range
15 - 35 - PDW, platelet distribution width at 20 of peak
height Reference range 9 - 14 fl
MPV (fl)
99Histograms
Thrombocyte Histogram Flags
Mark PL , abnormal height at lower
discriminator
PLT
Curve does not start from baseline
- Possible cause
- High blank value
- Cell fragments
100Histograms
Thrombocyte Histogram Flags
Mark PU , abnormal height at upper
discriminator
Curve does not reach baseline
- Possible cause
- Clotted blood sample
- EDTA-induced PLT clumping
- Micro-Erythrocytes
- Giant Platelets
- Platelet aggregates
101Histograms
Thrombocyte Histogram Flags
Mark MP , Multi Peaks found
- Possible Cause
- Platelet transfusion
102Histograms
Thrombocyte Histogram Flags
Mark DW , Distribution With
- The distribution can not be detected because the
Histogram does not cross the 20 limit twice. - This curve in only an example but could also show
another course.
103Histogram
Leukocyte-Histogram
Lyse of RBC and partial lyse of WBC
WBC
After lysis
Electrolyte solution
Mitochondria Nucleus Cell membrane Ribosome C
ytoplasm
104Histogramm
Leukocyte-Histogram
Before adding lysing reagent
Cell diameter in µm
Neutrophils Basophils Eosinophils Monocytes Lympho
cytes
10 - 15 9 - 14 11 - 16 12 - 20 7 - 12
0 2 4 6 8 10 12 14 16 18 20 22
After adding lysing reagent
Lymphocytes
MIX Monocytes Basophils Eosinophils
Neutrophils
0 50 100
150 200 250 300
105Histogram
Curve is limited by 2 discriminators.
Leukocyte-Histogram
- IMPORTANT
- The distribution curve should be within the
discriminators. The curve should start and end at
the basis line. - The LD is flexible, but can not be lower than 30
fl. - The WBC-channel show Leukocytes andThrombocytes
( Erythrocytes are lysed). - The volume of the Thrombocyts is usually between
8 - 12 fl, therefore the LD at the WBC-Histogramm
seperates the Leukocytes from the Thrombocytes.
(Thrombocytes were not counted).
106Histogram
Leukocytes-Histogram Flags
1. Flag WL , Curve does not begin at the basis
line
- Possible cause
- Thrombocytes EDTA-not compatible Probe
coagulated - high osmotic resistant (Erythrocytes not lysed)
- Normoblasten / Erythroblasten
- cold agglutinate
107Histogram
Leukocytes-Histogram Flags
2. Flag WU , Curve does not end at the base
line.
Sample dilution ? ( high leukocyte count ?
)
108Histogram
Leukocytes-Histogram Flags
3. Flag T1 and T2
T1 and T2 are trough discriminators defined by
the plateau. This discriminators separates the
Leukocytes populations.
- The discriminators are flexible and will be set
automatically according to the sample. - In special cases is a separation from the trough
discriminators not possible.
109Histogram
Leukocytes-Histogram Flags
3. Flag T1 and T2
- Attention
- Confirm the result with the microscope if T1 or T
2 flag was indicated. - The WBC result will be correct if no flag is
indicated. All Leukocytes are counted.
T1 could not be detected No plateau/trough was
found. --gt T 1 flag
T1 was detected but not T2 --gt T2 flag
110Histogram
Leukocytes-Histogram Flags
3. Flag F1 , F2 and F3
The Histogram of the Leukocytes is limited from
the outer discriminators LD and UD.
F1 F2 F3
- All Leukocytes are counted WBC total is
correct. (Assumption no other flags) - T 1 and T 2 were detected.
- Conspicuous is The troughs are away from the
basis line.
There is a potential of mixing populations. F 1
and F 2 move together. Also F2 and F3.
111Maintenance
Interpretation
Maintenance
112Maintenance
- 1. External Check
- Is there enough reagent for routine daily
analyses? CELLPACK ca. 750 Cycles / 20
lSTROMATOLYSER-3WP ca. 1.000 Cycles / 0,5
lSULFOLYSER ca. 1.000 Cycles / 0.5 l - Enough printing-paper?
- Tubing and wiring connected correctly?
- Sampler ready?(optional)
- Waste container empty?
113Maintenance
- 2. Switch on the instrument
- Main switch at the instrument.
- 3. Auto-rinse sequence is performed
- Automatic background checkWBC 0,3 x
103/µlRBC 0,02 x 106/µl HGB 0,1 g/dl
PLT 10 x 103/µl - 4. Instrument goes into READY status.
- 5. Measure control material
- - Eight Check for F-Series K-Series
114Maintenance
- 5. Standby mode (only if available)
- If you do not use the analyser for a certain
period of time the system will go automatically
into the standby mode. Recovering by pressing
START button. - 6. Execute shut down sequence
- Press SHUT DOWN / END.
- Put CELLCLEAN under aspiration pipette. (for
K-4500 press 1). After the ready indication is
turned off and a peep signal sounds 3 times
remove the CELLCLEAN. The system performs
automatic rinse cycles and the message Turn
Power Off will be announced. - Turn the Analyser off.
115Maintenance
- Daily
- Clean transducer and manometerProgram Shut
Down" - Check trap chambers and remove water
- Weekly
- Clean tray under SRV
- Clean waste chamberProgram clean waste
chamber (if available) approx. 20 min - Monthly
- Clean orifice
- Clean WBC/RBC-aperture
116Maintenance
- If necessary
- Clean sample rotor valve
- Clean rinse cup mechanism
- Remove aperture cloggingProgram clog removal"
- Remove obstinate clogs at apertures.Program
drain sampleClean aperture manual with
transducer glass-fibre brush and Cellclean. - Reset counter (if available) Program cycle
counter" - (Servicereport) !!
117Thank you for your attention !!