Research projects

1
In, out around the membrane
Tina Howard
2
Research projects

• Principal Investigator Steve Prince
• Co-workers Olivia Sleator
• Tina Howard
• Robert Cleverley
• Collaborators
• External collaborators

3
Olivia SleatorTina HowardLu-Yun LianAshraf
Kitmitto
Kir ion channels
4
PSD95 (SAP90)
Tina Howard Lu-Yun Lian
Postsynaptic density protein 95
5
MPSI - BBSRC SPoRT project
Structural Biology of Membrane transport
Rob Cleverley Bob Ford Jeremy Derrick Neil Isaacs
etc.
6
P-glycoprotein
Bob Ford R Callaghan Yvonne Jones

• Eukaryotic ABC transporter
• implicated in drug resistance
• Bacillovirus/insect cell
• expression system
• Affinity purification
• Knowldege of inhibitors and
• conformational substates

7
Bacterial Omps OpcA HmBr Jeremy Derrick
8
Neutron crystallography
Jeremy Derrick Peter Timmins

• Contrast matching hydrogen location at
• high-resolution.

9
Kir Channels
10
PSD95 Scaffold protein Postsynaptic density
protein 95 (PSD95/SAP90) is a member of MAGUK
family (membrane-associated guanylate kinase)
that oligmerises and clusters ion channels and
associated signalling machinery at excitatory
synapses in the brain .
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