G1

1
a
TA1R
TB7R
TC3R
TB2R
TC3F
TM0105
5/1833
3/1833
1/1833
0/1833
0/1833
0/1833
TB21R
TA3F
TB2R
b
G5
G3
G1
G4
G2
M3
M1
M6
M4
M2
M5
c
d
e
B22Not
c8
c16
TM0885
P1301
2/409
0/173
0/292
0/798
M7
M8
G7
G8
M9
f
Supplementary Figure 1
2004-02-15137B/Kawasaki /Suppl.Fig.1ppt
2
Supplementary Figure 1 Positional cloning of
CASTOR and POLLUX genes. a, Genetic map around
the CASTOR locus. Positions of flanking markers
are indicated together with the number of
recombinant plants/total number of mapping
individuals. b, Physical map of the CASTOR locus.
Designations of large insert clones originating
from parental ecotype B-129 are G1, BAC156-1E
G2, BAC124-7B G3, BAC324-1D G4, BAC104-3F G5,
BAC33-3E and from parental ecotype MG-20 M1,
LjT17M09 M2, LjT62O06 M3, LjT02K14 M4,
LjT45I15 M5, LjT20F11 and M6, LjT46G19. An
inverted region of 145kb between the genomes of
B-129 and MG-20 was detected, which is probably
responsible for the observed suppression of
recombination around the CASTOR locus (0 cM). BAC
end markers within the inverted region are open
oval, TB21R closed oval, TA3F open rectangle,
TB2R. The TB7R marker located in the north side
delimits CASTOR to 240kb. c, Features and
candidate genes predicted within the target
region. LRRPK, leucine-rich repeat receptor
kinase 26SP, 26S proteasome regulatory subunit
7 RE, retro-element EP, Avr9/Cf-9 elicited
protein PUM, pumilio-family RNA-binding protein
RHZF, Ring H2 zinc finger protein PME, pectin
methyl esterase IF2, initiation factor 2
subunit OXI, oxido-reductase PP, polyprotein
MYB, myb family protein GAG, gag-pol
polyprotein FIP VirF-interacting protein FIP1
ANK, ankyrin-like protein REV reverse
transcriptase unlabelled, hypothetical protein.
d, Exon-intron structure of CASTOR. Exons are
indicated by upper boxes and numbers indicate
their length in nucleotides. Introns are
represented by lower triangles. Several
alternative-splice variants were identified by
cDNA-sequencing a 4-nt insertion at the 3 end
of the 1st exon, and a 9-nt insertion at the 5
end of the 2nd exon. The resulting exon sizes are
shown in brackets. Also, the 7th intron of 365
nucleotides (365) was retained in 15 out of 17
cDNA clones, leading of a premature stop codon.
e-f Genomic region around the POLLUX gene e,
Genetic and physical map of the POLLUX locus.
Positions of the flanking markers and the number
of recombinant plants in the mapping populations
are indicated. Abbreviations G6, G7, M7, M8 and
M9 refer to large insert clones BAC131-3b,
BAC45-6C, LjT39N07, LjB22b22 and LjT45B09,
respectively. Genes predicted on the
cosegregating TAC clone LjT45B09 WD40, WD40
repeat protein NifU, putatively involved in Fe-S
cluster synthesis PPR, pentatricopeptide repeat
protein DNABP, DNA binding protein LRRPK,
leucine-rich repeat receptor kinase unlabelled,
hypothetical protein. f, Exon-intron structure of
POLLUX. One alternative splice site was detected
at the beginning of the 10th exon resulted in a
16-bp deletion.