Innovative component: Medium chain fatty acids

1
Innovative componentMedium chain fatty acids

• Caproic acid C6
• Caprylic acid C8
• Capric acid C10
• Lauric acid C12

2
Background MCFA

• Present in milkfat, coconut and palmkernel fat as
  triglycerides
• After technological refinery steps (purification,
  hydrolysis, distillation, purification) result
  MCFA
• Its use in animal feed is protected by worldwide
  patents
• Broad antibacterial activity, also Gram positive
  bacteria

3
Growth inhibition by caprylic acid on E-coli
ID-Lelystad, 2000
4
Growth inhibition by capric acid on E-coli
ID-Lelystad, 2000
5
Organic acids vs. MCFA Antibacterial effect
(equimolar basis)
De Cuypere et al, 1994
6
Antibacterial effects MCFAMIC test no pH
correction
Nutreco - ID Lelystad, 2000
7
In vitro metabolite production caecal microflora
Wageningen University (The Netherlands), 1999.
8
Metabolism of caecal flora as influenced by AMGP
or medium chain fatty acids (Finger printing)
Wageningen University (The Netherlands), 1999.
9
Effect MCFA on clostridium broilers
Broilers were infected with Eimeria spp. on day
10 and thereafter with Clostridium perfringens
vegetative cells on day 15 to 17. Clostridium
lesions were counted in the following days, as
well as performance over total growout period.


ID Lelystad, Netherlands, 2002
10
Proposed dual mode of action MCFA

• Normal situation ATP-dependent influx of H
  (barrier function)
• Invasion into cell in undissociated form (conform
  organic acids)
• Integration into cell wall, thereby creating
  leaks (H invasion), socalled Uncoupling
• ? Synergy between MCFA and organic acid (H)
  in bacterial killing!

After Stratford and Anslow, 1996.