CLVM

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Cell Line Vial Maintenance Program
CLVM
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What is CLVM Program? Who can use this? Why to
use this?
Let us know one by one..
What is CLVM Program?
This is a Cell Line Vial Maintenance (CLVM)
repository provides information about the cell
lines stored in liquid nitrogen cylinders. The
information includes locations of the vials
containing cell line of interest in various
cylinders, freezing records of the vials like
medium used for freezing, date of freezing, etc.
and conditions used for maintenance of the cell
line. Moreover, listing of the stored cell lines
can be obtained on the basis of cell type, tissue
of origin and species origin. It also indicates
the empty positions available for storing
freezing vials.
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Who can use CLVM Program?
Any laboratory where tissue or cell culture
work is a routine activity and needs to keep a
track of stock for longer period can use this
CLVM software.
Why to use CLVM Program?
Traditionally, records of frozen vials of various
cell lines are maintained in the files or record
books. To search for a particular vial of
interest or to check the earlier records for
comparison or for stock checking, one has to
screen through several pages of the book. This is
very tedious and time consuming. CLVM saves time
and efforts and provides more information.
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Now let us check the programme..
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Cell Line details will cover

• Cell line Name and User
• Type, Tissue, Origin, Media, Percentage of Serum,
  Antibiotics, Conditioned media if any etc.
• Freezing details Date, Freezing media, serum,
  User, Passage/batch, Special remarks, etc.
• Actual location details of Particular Vial in
  Cylinder, Canister, Cane/Box along with its
  position number.

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Important Modules Data Entry for New Cell Line
along with Vial position details Cell Line
Revival Various Reports Stock frozen vials
positions - cell line wise Archival Records for
Revived cell lines Finding empty
positions/Occupied positions in Cane/Box List
of Cell lines Name/Type/Tissue/Origin wise
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ADD MODULE
This module handles data entry. The data includes
names of the cell line and user, detailed
information about the cell line, i.e., cell type,
tissue and species, maintenance and freezing
media etc. Most of the information can be added
by drop down choose menu. This reduces data entry
work. If multiple vials of one cell line are
prepared on the same date by the same user, then
option is provided to choose the cylinder,
canister and position for multiple vials.
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REVIVAL MODULE
Revival module provides help for revival of a
particular cell line. Location of the vial and
detailed information of the desired cell line can
be precisely checked. Once the chosen vial is
marked by entering revival date i.e. Rev. dt
and Revived by, it updates record such that the
marked vial position is now shown empty to add
new one.
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Revival module
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Other applications

• Cell Line Repositories
• Tissue Culture Laboratory
• Blood Bank

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• Advantages of programme
• Restricting Unauthorized Access
• Representing Data as per requirements e.g.
  variety of lists
• Controlling Redundancy
• Providing Backup
• Reduced Record Maintenance Time
• Standard can be enforced
• Flexibility
• Availability of up-to date information

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THANK YOU
Contact details for this Programme Advanced
Centre for Treatment, Research
Education(ACTREC) Tata Memorial Centre, Sector
22, Kharghar, Navi Mumbai 410208 Tel[phone]
Fax [phone] E-mailnnaik_at_actrec.gov.in