Title: Frequency of Tandem Repeats in Cyanobacteria
1Frequency of Tandem Repeats in Cyanobacteria
Nostoc punctiforme
Anabaena PCC 7120
2Tandem Repeats in CyanobacteriaWhat are they?
Total observed heptamers in chromosome 1110
Total types of heptamers
1170
If random, predict 1 observed heptamer per type
3Observed Number of Repeats per Type
Heptameric / octomeric repeats come in families
4Observed Number of Repeats per TypeMajor types
Anabaena Nostoc
Family Rank Observed Rank Observed
STRR1 AATCCCC 3 101 18 21 AGTCCCC 4 69 23 15 AT
TCCCC 25 9 5 58
AdTCCCC 179 94
Nomenclature and first observation by Mazel
et al (1990) J Bacteriol 1722755-2761
5Observed Number of Repeats per TypeMajor types
Anabaena Nostoc
Superfamily Rank Observed Rank Observed
STRR1 AdTCCCC 179 94 STRR2 AATGACh 80 290 ACT
GACh 141 4 STRR7 AAAATyy 121 78 STRR9 ArGGGTGT
119 1 STRR5 AATTACG 17 65 STRR6 AGCAGGGG 8
59 STRR8 ACTChTA 1 65
6Location of heptamers/octamers in genome
Number of hepta-/octamers
1 gt6
chromosome 6414 kb
7Location of 9-mers/12-mers in genome
Number of 9-/12-mers
1 gt6
chromosome 6414 kb
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9MiniTransposon in genome of Anabaena
Transposon in genome of Anabaena
Iterated sequence in genome of Anabaena
Strr9 direct
Strr9 complement
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11Alignment of NIS-1
(271 more)
12Filters Information reducersBuild secondary
repeats filter
13Filters Information reducersBuild secondary
repeats filter
Distance 2
14Filters Information reducersBuild secondary
repeats filter
B CTTGTACTGAGCGTAGCCGAAGTA C GTTCGACTGAGCGTAGTCG
AAGTC
Do for all pairs of subfamilies
15Relationship between related repeats in
genome(sequences within NIS-1 repeat family)
Diameter Copies of exact repeats
Distance Number of mismatches
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