Instruments of Bacteriology

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Instruments of Bacteriology

• Proper Operating Procedures

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Proper Bacteriology Lab Procedure

• Assume all bacteria are pathogenic.
• Pathogen Any organism that can cause disease.
• Some bacteria.
• Some protists.
• Some fungi.
• Some viruses.

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Proper Bacteriology Lab Procedure

• Assume all bacteria are pathogenic.
• Assume the previous student did not follow proper
  procedures.
• Wipe area down with antiseptic.
• Perform lab. Follow sterile procedure (aseptic
  technique).
• Dispose of all material properly.
• Wipe area down with antiseptic.
• Wash and dry hands.

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Commonly Used Equipment

• Autoclave
• Incubator
• Bunsen Burner
• Inoculating loop
• Petri dish
• Agar

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Autoclave

• Sterilization
• Heat (215 degrees F)
• Pressure (15 lbs/in2)
• Kills all
• (vegetative spores)

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Large Hospital Autoclave
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Veterinary Autoclave
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Incubator

• Optimum conditions
• Temperature
• Moisture
• Dark
• Food
• Oxygen (or no oxygen)

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Inoculating Loop

• Metal

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Inoculating Loop

• Disposable (plastic)

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Bunsen Burner
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Bunsen Burner Flame
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Gas Valve

• Off
• On

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Flaming a Loop
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Petri Dish
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Agar

• Provides food (amino acids, carbohydrates)
• Water
• Surface area
• Differentiatial agar

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Contaminated Petri Dish

• Incomplete pour-left uncovered for _at_ a ½ hour.

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Bacterial Colonies

• Colony contains
• millions of bacteria
• One colony started
• with ?

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Streaking a Plate
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Colony Characteristics