HyperChem Model of Human Chromosome 21q22'3 Gene WDR4 DNA,

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HyperChem Model of Human Chromosome 21q22.3 Gene
(WDR4) DNA, mRNA, and Protein
Adrienne Tveit Chem 451 September 15, 2000
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Human Chromosome 21q22.3 Gene (WDR4) clone
DNA Sequence atg gcg ggc tct gtg gga ctg gcg ttg
tgc ggg cag acg ttg gtg gtg cgg
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Double Stranded DNA
Hydrogen Bond
Purine
Pyrimidine
Major Groove
Minor Groove
Deoxyribose
Phosphorus
Oxygen
DNA Sequence shown atgg
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mRNA Complimentary to Human Chromosome 21q22.3
Gene (WDR4) cDNA clone
DNA Sequence (antisense strand) atg gcg ggc
tct gtg gga ctg gcg ttg tgc ggg cag acg ttg gtg
gtg cgg
DNA Sequence (sense strand) tac cgc ccg aga cac
cct gac cgc aac acg ccc gtc tgc aac cac cac gcc
mRNA Sequence aug gcg ggc ucu gug gga cug gcg
uug ugc ggg cag acg uug gug gug cgg
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Single Stranded RNA
Ribose
OH on C2
Phosphate group
Ribose
Phosphate
Oxygen
Ribose
Phosphate group
RNA backbone pattern ribose, phosphate, ribose,
phospate, etc.
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Primary Structure of Peptide Coded for by the
First 51 Nucleotides of Human Chromosome 21q22.3
Gene (WDR4) cDNA clone
Codon Amino Acid aug Start gcg Alanine ggc Glycine
ucu Serine gug Valine gga Glycine cug Leucine gcg
Alanine uug Leucine ucg Serine ggg Glycine cag Gl
utamine acg Threonine uug Leucine gug Valine gug V
aline cgg Arginine
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Description of Protein Function
The isolation and characterizaiton of human
chromosome 21q22.3 Gene (WDR4) codes for a
WD-Repeat protein that may be able to identify
candidaste genes for Down Syndrome phenotypes.
Trapped exons are being used to identify the
repeat protein that encode the same 412 amino
acid protein repeats. Additional splicing events
among the protein were abserved, where it is
possible for several genetic disorders could be
used in association studies.
Translation of Amino Acid Code for Human
Chromosome 21q22.3 Geme (WDR4)
MAGSVGLALCGQTLVVRGGSRFLATSIASSDDDSLFIYDCSAAE
KKSQENKGEDAPLDQGSGAILASTFSKSGSYFALTDDSKRLILFRTKPWQ
CLSVRTVA RRCTALTFIASEEKVLVADKSGDVYSFSVLEPHGCGRLKLG
HLSMLVDVAVSPDDRFI LTADRDEKIRVSWAAAPHSIESFCLGHTEFVS
RISVVPTQPGLLLSSSGDGTLRLWEY RSGRQLHCCHLASLQELVDPQAP
QKFAASRIAFWCQENCVALLCDGTPVVYIFQLDAR
RQQLVYRQQLAFQHQVWDVAFEETQGLWVLQDCQEAPLVLYRPVGDQWQS
VPESTVLK KVSGVLRGNWAMLEGSAGADASFSSLYKATFDNVTSYLKKK
EERLQQQLEKKQRRRSP PPGPDGHAKKMRPGEATLSC
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References
Michaud J, 2000, Isolation and Characterization
of a Human Chromosome 21q22.3 Gene (WDR4) and Its
Mouse Homologue That code for a WD-Repeat
Protein. Genomics, 68, pp 71-79. Voet D, Voet JG,
and Pratt CW, 1999, Fundamentals of Biochemistry.
John Wiley sons, New York, NY, pp 165-167.