Exam 3 (mean 76%)

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Exam 3 (mean 76)
A 70-63
B 62-56
C 55-49
D 48-42
F 41-0
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• Today-Ch 13
• Announcements
• Look for Chapter 12 and 13 quizzes posted this
  weekend.
• Exam 4 covers Chapters 12, 13 and 16 (last exam
  you can drop. Everyone must take the final exam)

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Biology 190Chapter 16 pictures
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DNA Replication occurs at the S stage of mitosis
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Fig 5.26 Nucleotides
U
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Fig 16.5 One DNA strand.
A C C C C A G
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Fig 16.6 Double Helix
A C C C C A G
-T -G -G -G -G -T -C
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Chargaffs Rule
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Question 16.1
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Functions of Proteins

• Structural
• Catalytic activity
• Immunity
• Regulation
• Poisons
• Transport molecules
• Hormones
• Fluid and electrolyte balance
• Sources of energy

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Fredrick Griffiths Experiments
Polysaccharide Coat
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Fredrick Griffiths Results
Live R-strain picked up DNA from the S-strain.
Contains genes for smooth coat.
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Alfred Hershey and Martha Chase
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How viruses (phages) infect bacteria
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Fig 16.4 Hershey and Chase
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Watson and Crick
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Rosalind Franklins Data(Maurice Wilkins Lab)
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What exactly IS the structure of DNA?
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Chargaffs Rule
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Nucleotide base pairing
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Fig 16.7 Structure of DNA
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Nobel Prize- 1962
Wilkins
Crick
Watson
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Figure 16.9 Semiconservative Model for DNA
Replication
Parental strands (template strands)
Daughter strands
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DNA Origin of Replication
Bacteria have one origin of replication.
Fig 16.12 Eukaryotic organisms have
multiple origins of replication.
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Unwinding DNA
Single-stranded Binding Proteins
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Nucleoside Triphosphate
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Fig 16.13 DNA Polymerase
(Old strand)
(Daughter strand)
(Parent strand)
P
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Questions 6.3 6.4
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Fig 16.14
Leading Strand
3
5
Old strands Parent strands Template strands
Replication Fork
5
3
5
3
3
Lagging Strand
5
3
5
3
5
5
3
3
5
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Fig 16.14
DNA Polymerase III
DNA Ligase
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Fig 16.13 DNA polymerase needs a 3 -OH group
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Fig 16.16
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Fig 16.16. Review of Bacterial DNA Replication
SS binding proteins
Helicase
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Fig 16.18DNA shortening and telomeres
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Mismatch repair
2 Purines bulge
G
A
2 Pyrimidines dimple
T
C
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Fig 16.17 Excision Repair
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Xeroderma Pigmentosa
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In very, very, vary rare cases mutations have a
selective advantage