Pickering Laboratories Corporation

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Pickering Laboratories Corporation

• Post-Column
• Instruments and Chemistry

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Guaranteed Chemistry

• Only Pickering Laboratories Makes the Chemistry,
  Columns and Systems specifically optimized for
  each analytical Method.
• Guaranteed to produce excellent Chromatography
• Reproducible, Sensitive and Specific

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Post-column Derivatization

• Renders visible certain compounds that are
  normally invisible by performing a chemical
  reaction on the substances that gives them an
  easily detectable physical property
• Reaction typically produces a strong color or
  fluorescent product
• Used to increase sensitivity and selectivity in
  HPLC analysis
• Mixes the stream of effluent flowing from the
  HPLC column with a stream of reagent solution
• Compounds and reagent flow into reaction chamber
• Typically heated
• Some methods require two (or even three)
  reactions in series
• Derivatized compounds pass into a detector

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Post-Column Analysis Methods (Partial List)

• Aminoglycosides Antibiotics
• Amino Acids
• Biogenic Amines
• Bromate
• Carbamate Pesticides
• Chromium VI
• Formaldehyde
• Glyphosate Herbicide

• Guanidinos
• Hexosamines
• Mycotoxins
• Paralytic Shellfish Toxins
• Paraquat Diquat
• Polyether Antibiotics
• Sulfa Drugs
• Transition/Rare Earth Metals

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US EPA (Environmental Protection Agency)
Regulations Specify Pickering Laboratory

• Drinking water regulation 531.2 testing for
  Carbamates Specify Pickering chemistry and
  methods
• Water regulation 547 testing for Glyphosate
  Specify Pickering chemistry and methods

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AOAC international (Association of Analytical
Communities) Official Validation

• AOAC Official Method 985.23 N-Methylcarbamates
  Insecticide and Metabolite Residues
• AOAC Official Method 991.08 Glyphosate and
  Aminomethylphosphonic Acid (AMPA) in
  Environmental Water
• AOAC Official Method 994.12 Amino Acids in Feeds

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PCX5200 Post-Column Derivatization Instrument

• Rugged
• Reliable
• Optional PC Software
• Column safeguards
• Low-pulsation
• Reactor Temp to 130º C

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Post-Column Reagents

• Optimized for Post-Column Methods
• Chromatographic Grade
• 99.9 Purity
• Consistency
• Pre-mixed for laboratory efficiency

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Columns and Guards

• Optimized for Post-Column Methods
• Quality Assurance Individually Tested
• Reliability
• Longevity
• Laboratory Cost effectiveness

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Pickering Labs.Com
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Company

• 23 Years of business
• Direct Sales in US and through 87 international
  distributors
• Headquarters in Mountain View California, USA