rough sheath2 rs2

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rough sheath2 (rs2)

• By Charlene Kormondy and Sarah Higbee-Tindell

rough sheath2 (rs2), CMK, SRH, 1-13-05
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Table of Contents

• Information
• Whats wrong with the mutant
• DNA sequence
• DNA sequence
• explanation
• References

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Information

• Bass lab family number 1558
• The rs2 gene has been cloned
• The gene is on chromosome 1
• The mode of inheritance is recessive

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What is wrong with the mutant?

• The rs2 mutant has warty distorted leaf blade
  sheath.

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DNA sequence of rs2
Zea mays rough sheath2 (rs2) cDNA gene
sequence gtgi4583430gbAF126489.1AF126489
      1 CGCGCATTAT AAGGTGTGGT GGTGAGGGAT GGGCGATG
GC CTTTGCAGAT GAAGGAGCGA        61 CAGCGCTGGC GGC
CTGAGGA AGACGCCGTC CTGCGCGCCT ACGTCCGGCA GTACGGCCC
C       121 CGCGAGTGGC ACCTGGTGTC GCAGCGCATG AACG
TGGCCC TCGACCGCGA CGCCAAGTCG       181 TGCCTGGAGC
 GCTGGAAGAA CTACCTCCGC CCCGGGATCA AGAAGGGCTC GCTCA
CCGAG       241 GAGGAGCAGC GCCTGGTGAT CCGCCTGCAG 
GCCAAGCACG GCAACAAGTG GAAGAAGATC 
     301 GCCGCCGAGG TGCCCGGGCG CACGGCCAAG CGCCTCGG
CA AGTGGTGGGA GGTGTTCAAG       361 GAGAAGCAGC AGC
GGGAGCT GAGGGACAGC CGCCGCCCGC CGCCGGAGCC TAGCCCCGA
C       421 GAGCGGGGCA GGTACGAGTG GCTGCTCGAG AACT
TCGCGG AGAAGCTCGT CGGAGAGAGG       481 CCACAGCAGG
 CTGCCGCTGC GCCGTCGCCG CTGCTCATGG CCGCGCCCGT GCTGC
CGCCC       541 TGGCTGTCGT CCAACGCCGG GCCGGCCGCC 
GCCGCCGCCG CCGCCGTCGC GCACCCGCCG 
     601 CCCAGACCGC CGTCGCCGTC CGTGACGCTC AGCCTCGC
GT CCGCCGCGGT GGCGCCGGGC       661 CCGCCCGCGC CCG
CGCCATG GATGCCGGAT CGCGCCGCGG CCGACGCGGC GCCGTACGG
G       721 TTCCCGAGCC CGTCGCAGCA CGGCGGCGCC GCGC
CCCCGG GCATGGCGGT CGTGGACGGG       781 CAGGCGCTGG
 CGGAGCTCGC CGAGTGCTGC CGCGAGCTGG AGGAGGGGCG GCGCG
CGTGG       841 GCGGCGCACC GGCGGGAGGC GGCGTGGCGG 
CTGAAGCGCG TGGAGCAGCA GCTGGAGATG 
     901 GAGCGCGAGA TGCGGCGGCG CGAGGTGTGG GAGGAGTT
CG AGGCCAAGAT GCGCACCATG       961 CGGCTGGAGC AGG
CGGCCGC CGCGGAGCGC GTGGAGCGGG ACCACCGGGA GAAGGTGGC
C      1021 GAGCTCCGCC GCGACGCGCA GGTCAAGGAG GAGA
AGATGG CCGAGCAGTG GGCCGCCAAG      1081 CACGCGCGCG
 TCGCCAAGTT CGTCGAGCAG ATGGGGGGGT GCTCCCGTTC ATGGT
CCTCT      1141 GCCACCGACA TGAACTGTTG AACTGGACAA 
ACGAGCCGCT GACCACACCG TCGCTAGCTA 
    1201 TCGCGTGCTA CGTGCTCCGG TTCACGGCTT TCTTGCAT
TC TGGGACGCGA CTTGTAAACG      1261 CCATGGATTC CCT
TGCTCAC GTTCACTTCC GCTTTCTTTT TTTCTTTTCT TTTTATTTA
C      1321 AACTGTTGTT ACGGTCGGTT CAATGCTTCT CTCT
CTATCT ACTAGTAGTT GATCAGAGTC      1381 AAGAAATGTA
 ATGCTGCCGT TACTGTCGTA GTTTCATCCT  
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DNA sequence Explanation

• DNA DeoxyriboNucleic Acid
• A Adenine
• G Guanine
• C Cytosine
• T Thymine
• A T go together
• G C go together
• First strand GCTA
• Second strand CGAT

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Reference

• Hadjinov, M.I. (1937)
• Genes of Rough Sheath in maize.
• Bull Appl Bot Gen Plant Breed Ser II 7247-258.

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Thanks to

• Dr. Hank Bass
• Ms. Debbie Figueroa, graduate student
• Ms. Bobbye Hill, biologist
• Dr. Brian Ring, post-doctoral research associate
• And of course, the wonderful Mrs. Crow

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