SAMPLING METHOD ACCORDING TO THE GOAL

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SAMPLING METHOD ACCORDING TO THE GOAL
AVIAN MYCOPLASMAS PCR
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Which sample?
Samples must be representative of the flock and
of the pathology Samples must be likely to
contain the micro-organisms It s important to
sample individualy Samples must be refrigerated
to prevent DNA lysis
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Which sample?
On live birds Tracheal swabs Cloacal
swabs Palatine slit swab of DOP At necropsy Air
sacs Joint, articular liquid embryonated
eggs Vitellus Environment Building walls or
material drag-swabs, dust
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How many sample?
The number of swabs depends on

• The expected prevalence
• The level of confidence
• The flock size
• is given by the following formula
  (1-(1-C)1/d)x(N-(d-1)/2)

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Sampling size?
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Cost analysis
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Cost analysis
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Cost analysis
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Cost analysis