Presentacin de PowerPoint

1
NATIONAL DNA BANK
BIOSPAIN, Barcelona, 2004
2
GENOME SPAIN INITIATIVE
Genoma España

• The National DNA Bank is a technological
  platform created earlier this year in order to
  provide services to the Spanish scientific and
  healthcare communities.
• To promote and further support research into
  genetic/genomic diversity with special emphasis
  on the population living in Spain.

General Aim
3
NACIONAL DNA BANK GENERAL GOAL

• To promote and further support research into
  genetic/genomic diversity associated with HEALTH,
  disease pathogenesis and therapy and human
  evolution, with special emphasis on the
  population living in Spain.

• To identify genes in complex diseases.
• To disect the role of environmental, social,
  nutritional and lifestyle factors in complex
  diseases.
• To promote research in pharmacogenomics
  (interaction between genes, phenotype and drug
  response).

4
NATIONAL DNA BANK Specific objectives

• Collection of samples from healthy volunteers
  (reference population).
• Storage of the samples.
• Sample cell line immortalization.
• Collection of samples at disposal of
  researchers.

Phase 1 general reference population (n1300)
(network of regional blood transfusion centers,
RBTC) Phase 2 patientspopulation with illness
of clinical relevance (research networks).
5
NATIONAL DNA BANK Structure
GENOME SPAIN
December 2003
UNIVERSITY of SALAMANCA
July 2003
NATIONAL DNA BANK
6
NATIONAL DNA BANK Structure
DEPARTMENT OF HEALTH Junta de Castilla y León
GENOME SPAIN
December 2003
March 2004
UNIVERSITY of SALAMANCA
July 2003
NATIONAL DNA BANK
7
NATIONAL DNA BANK Personal resources
Research Team Dr. E. de ALAVA Prof.
A. DOMINGUEZ Prof. R. GONZALEZ SARMIENTO Dr. J.
HERNANDEZ RIVAS Prof. E. SANTOS Prof. A. ORFAO
DIRECTION AND COORDINATION Director Prof.
Alberto Orfao Vice-director Dr. Enrique de
Álava Coordinator Dr. Andrés García Montero
SECRETARY Ana Mª Regalado
Laboratory unit
Storage unit
Computer unit
8
NATIONAL DNA BANK Structure
DEPARTMENT OF HEALTH Junta de Castilla y León
GENOME SPAIN
UNIVERSITY of SALAMANCA
SAMPLE INPUT
NATIONAL DNA BANK
REGIONAL TRANSFUSION CENTERS (n 30)
RESEARCH NETWORKS
9
NETWORK OF REGIONAL TRANSFUSION CENTERS AND BLOOD
BANKS
REGIONAL TRANSFUSION CENTER
BLOOD BANK
10
NETWORK OF REGIONAL TRANSFUSION CENTERS AND BLOOD
BANKS
REGIONAL TRANSFUSION CENTER
BLOOD BANK
11
RTC/BB DNA bank Genotyping
center
Blood samples
Separación celular y extracción de ADN
Sample storage
Voluntary donors
Reception/shipment of samples
Genotyping studies (SNPS)
Encoding
Database
Questionnaire about health, nutrition,
lifestyle,, etc
12
COLLECTION OF SAMPLES
Regional Transfusion Centres (RTC)
Voluntary donor
Information (RTC)
LIMS
Sample tracking
Informed consent
20 ml EDTA
Blood bank code
Blood extraction
10 ml Heparin
Shipment to DNA Bank
Questionnaire
- Health - Nutrition and lyfestyle - Demographic
questionnaire - Genealogical information
13
RTC/BB DNA bank Genotyping
center
Blood samples
Separación celular y extracción de ADN
Sample storage
Voluntary donors
Reception/shipment of samples
Genotyping studies (SNPS)
Encoding
Database
Questionnaire about health, nutrition,
lifestyle,, etc
DONATION DNA BANK
ANONYMIZED CODE CODE
CODE
14
SAMPLE PREPARATION STORAGE DNA Bank
EDTA Blood
Heparinized Blood
Extracted DNA (Gentra kit)
Whole blood
Leukocytes
Plasma
Automatic liquid handling aliquoting
Immortalized cell lines
STORAGE
2D-coded microtubes (Micronic) at -80ºC
FTA paper (RT)
Liquid N2
15
RTC/BB DNA bank Genotyping
center
Blood samples
Separación celular y extracción de ADN
Sample storage
Voluntary donors
Reception/shipment of samples
Genotyping studies (SNPS)
Encoding
Database
Questionnaire about health, nutrition,
lifestyle,, etc
DNA BANK L.I.M.S.
16
DNA BANK L.I.M.S. ACCESS FRONT PAGE
17
DNA BANK L.I.M.S. REGISTRY OF THE SAMPLE
ALIQUOTING PROCESS
18
DNA BANK L.I.M.S. INFORMATION ABOUT STORED
ALIQUOTS
19
NATIONAL DNA BANK Structure
GENOME SPAIN
DEPARTMENT OF HEALTH Junta de Castilla y León
UNIVERSITY of SALAMANCA
CEGEN
NATIONAL DNA BANK
RESEARCH NETWORKS (ISCiiiMSC)
REGIONAL TRANSFUSION CENTERS (n 30)
RESEARCH NETWORKS
INDIVIDUAL RESEARCH GROUPS
NATIONAL RESEARCH INSTITUTES
20
NATIONAL DNA BANK Structure
Advisory Committee on Ethics,...
External Scientific Committee
GENOME SPAIN
DEPARTMENT OF HEALTH Junta de Castilla y León
UNIVERSITY of SALAMANCA
CEGEN
NATIONAL DNA BANK
RESEARCH NETWORKS (ISCiiiMSC)
REGIONAL TRANSFUSION CENTERS (n 30)
RESEARCH NETWORKS
INDIVIDUAL RESEARCH GROUPS
NATIONAL RESEARCH INSTITUTES
21
NATIONAL DNA BANK Structure
Advisory Committee on Ethics,...
External Scientific Committee
GENOME SPAIN
DEPARTMENT OF HEALTH Junta de Castilla y León
UNIVERSITY of SALAMANCA
CEGEN
NATIONAL DNA BANK
RESEARCH NETWORKS FIS
REGIONAL TRANSFUSION CENTERS (n 30)
RESEARCH NETWORKS (n11)
INDIVIDUAL RESEARCH GROUPS
NATIONAL INSTITUTES OF RESEARCH (CNIC)
22
Advisory Committee of Experts on Ethical,
Economic, Environmental, Legal and Social Issues
Prof. Dr. Rogelio González Sarmiento Department
of Medicine (University of Salamanca) Drª.
Pamela Sankar Center for Bioethics (University of
Pennsylvania, USA) Prof. Drª. María
Casado Observatorio de Bioética y Derecho
(University of Barcelona)
Prof. Drª. Bartha Knoppers University of Montreal
(Canada) Prof. Dr. Carlos María Romeo
Casabona University of Deusto University of
País Vasco/EHU Prof. Dr. José Román
Flecha University Pontificia of Salamanca Prof.
Dr. Juan Ramón Lacadena Calero Deparment of
Genetics, Faculty of Biology (University
Complutense of Madrid)
23
External Scientific Committee
Dr. Jaume Bertranpetit, National Genotyping
Centre, University of Barcelona. Prof. Alberto
Orfao, National DNA Bank University of
Salamanca. Dr. Elías Campo, Servicio de Anatomía
Patológica del Hospital Clinic i Provincial,
Barcelona. Prof. Julio Benítez, Department of
Psichiatric Pharmacology, University of
Extremadura. Dr. Jaume Marrugat, Instituto
Municipal Investigación Médica, Barcelona. Dr.
Rubén Moreno, Fundación Valenciana de
Investigaciones Biomédicas (FVIB),
Valencia. Prof. Carlos López Otín, Department of
Molecular Biology, University of Oviedo.
Dr. Thomas Hudson, Genome Quebec Innovation
Centre McGill University, (Canada). Dr. Yusuke
Nakamura, Human Genome Center, Institute of
Medical Science, University of Tokyo (Japón). Dr.
Pui-Yan Kwok, Cardiovascular Research Institute,
University of California (USA). Dr. David
Bentley, The Sanger Institute (UK). Prof. Peter
Donnelly, Department of Statistical Science,
Oxford University (UK). Prof. Mark Lathrop,
Centre National de Genotipage, (France). Prof.
Bill Ollier, Centre for Integrated Genomic
Medical Research, University of Manchester (UK).
24
THANK YOU
Secretary Banco Nacional de ADN Centro de
Investigación del Cáncer (USAL) Paseo de la
Universidad de Coimbra s/n 37007 Salamanca Tel
923294833 Fax [phone] E-mail bancoadn_at_usal.es
25
NATIONAL DNA BANK International Structure
GenomEUTwin Project
Estonian Biobank
P3G (Public Population Project in
Genomics) Harmonizacion of Biobanks and
population-based cohorts
Cartagene/ Genome Canada
UK Biobank
National DNA Bank National Genotyping
Centre (Genome Spain)
26
NETWORK OF REGIONAL TRANSFUSION CENTERS AND BLOOD
BANKS