Cellular Aging and Cellular Immortality

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Cellular Aging and Cellular Immortality

• The Study of Cells
• In vitro

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Nature vs. Nurture Question?? Nature-
Preprogrammed, Inherited Senescence Nurture-
Change in Environment
How to Answer Question Transplantation- eg. pig
skin Cell Culture
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The Hayflick Limit
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Is There a Connection Between Organismal Aging
and Cellular Aging ??
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Structural / Functional Changes of
Non-Proliferating Cells
1. Increase lysosomes 2. Irregularly lobed
nucleus 3. Abnormal mitochondria 4. Increase in
Cell Size 5. Decrease in RER, ribosomal RNA
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Cellular Immortality Cell Lines eg.
HeLa Transformation- Retrovirus eg. Rous
Sarcoma Virus Big T-antigen (SV-40) Telomerase
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CHO Cells in Culture