Neuropharmacology Methods

1
Neuropharmacology Methods

• Stereotaxic Surgery
• Quantification/Localization of Proteins
• Neuroimaging
• Genetic Manipulation
• Behavioral Pharmacology

2
Stereotaxic Surgery

• Lesioning/Microinjection
• Electrolytic lesions
• Neurotoxic lesions
• Microdialysis

3
Human Injuries
4
Stereotaxic Surgery

• In vivo voltammetry
• Electrophysiology
• Macroelectrodes
• Microelectrodes
• Patch-clamp

5
Protein Quantification

• Radioligand Binding
• Receptor autoradiography
• In vivo receptor binding

6
Protein Quantification

• Assays of enzyme activity
• Antibody production
• Immunocytochemistry
• Radioimmunoassay
• In situ hybridization
• DNA microarrays

7
Neuroimaging

• Computerized Tomography (CT)
• Magnetic Resonance Imaging (MRI)
• Positron Emission Tomography (PET)

8
Neuroimaging

• Single-photon Emission Computerized Tomography
  (SPECT)
• Functional MRI (fMRI)
• EEG

9
Genetic Engineering

• Targeted mutations/Knock-outs
• Gene replacement/Transgenics
• Antisense nucleotides
• reversible

10
Behavioral Pharmacology

• Evaluation
• Validity and Reliability
• Oversight

11
Behavioral Pharmacology

• Simple Observation
• Motor Activity
• Analgesia
• Learning and Memory
• Delayed-response test

12
Behavioral Pharmacology

• Measures of Anxiety
• Light-dark crossing
• Elevated plus maze
• Measures of Fear
• Conditioned emotional response
• Potentiated startle response
• Measures of Reward
• Conditioned place preference

13
Behavioral Pharmacology

• Operant Methods
• Measuring Anxiety
• Conflict test
• Measuring Drug Reward/Reinforcement
• Self-administration
• Negative Reinforcement
• Operant analgesia test
• Learned helplessness