Portable HighThroughput Integrated Laboratory Identification System PHILIS

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Portable High-Throughput Integrated Laboratory
Identification System (PHILIS)

• Building Environmental Analytical Capability for
  Chemical Warfare Agents in Support of EPAs
  Emergency Response Missions

Larry Kaelin National Decontamination Team US EPA
Special Teams
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PHILIS 1

• 2004 - Originally, DHS-led initiative to produce
  a high-throughput screening system (original
  goal 1000 samples/24 hrs) designed to identify
  and quantify Toxic Industrial Chemicals (TICs)
  and CWAs above permissible exposure limits (PEL)
• Field Testing of 3 Prototype Designs completed
  July 2005
• All vendors failed field test in both number of
  samples and accurate identification of compounds
• EPA expressed concerns that we did not wish to
  sacrifice quality for quantity
• Department of Homeland Security (DHS) and EPA
  signed MOU for transfer residual PHILIS-1
  equipment, and the development of new PHILIS 2
  system
• PHILIS 1 assets used to construct PHILIS 2
  prototype mobile laboratories PHILIS 2
  completed and delivered to EPAs National
  Decontamination Team (NDT) on April 2008

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Migrate PHILIS 1 to PHILIS 2

• The best aspects of the three PHILIS 1 systems
  were used to construct the new PHILIS 2 prototype
  (PHILBERT)
• GC/MS w/FPD
• Pressurized solvent extraction sample prep (PSE)
• LC/MS-MS added
• LIMS
• The remaining PHILIS 1 assets were used as a
  starting point to construct new PHILBERT systems
  (pending in NJ and CO)
• 10 GC/MS systems (NJ)
• 1 LC/MS-MS added (NJ)
• 2 - P T systems added (NJ)
• 3 TOF GC/MS systems (CO)
• SPE sample prep (CO)

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PHILIS 1 - NJ

• PHILIS 1 vehicles arrived in Edison, NJ in July
  2007. Currently running under temporary shore
  power and shelter.
• OEM will be responsible for operation and
  maintenance of PHILIS units, Region 2 providing
  warehouse/office space.
• Renovations to warehouse to begin November 2008
• Currently capable of only analyzing TICs (method
  8260/8270)
• Edison unit includes 10 GC/MS instruments and a
  sample prep trailer. Two P T, LC/MS-MS
  recently (October 2008) added.
• A Sample Log-in Area (SLA) trailer needed
• NDT will work with ERT and Region 2 emergency
  response program and laboratory on initial set up
  of lab.

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NJ PHILIS
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NJ PHILIS 1 of 2 Lab Trucks
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PHILIS Status - NJ

• Have Modified PHILIS 1 Systems
• 2 24 bed lab trucks (APL01-VOCs, APL02-SVOCs)
• 1 24 bed 5th wheel trailer w/Ford 550 for
  sample prep (SPA)
• 1 12 bed refrigerated box truck
• 10 GC/MS systems
• 2 PT systems for VOCs
• 1 LC/MS-MS system (not in PHILIS, in Region 2
  fixed laboratory)
• Need - Permanent shore power and shelter solution
• Support offices, storage, meeting/training rooms
• Automated sample prep for SVOCs
• HVAC renovations as per HS requirements (if
  required)
• Link in with Region 2 and EPAs Lab Response
  Corps
• SLA trailer w/UDA CWA standards secure room
• LIMS upgrade

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PHILIS 1 CO

• Denver PHILIS unit includes sample prep trailer
  and mobile laboratory bus with 3 time of flight
  (TOF) GC/MS systems.
• OEM will be responsible for operation and
  maintenance of PHILIS unit.
• Warehouse lease procurement currently underway
• Projected move date to CO warehouse January 2009,
  linking with NCERT for shared warehouse/office
  space
• Currently capable of only analyzing TICs
• TOFs have potential to accelerate analysis time.
  Rapid TOF methods currently being developed and
  validated by NHSRC and NEIC

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CO PHILIS
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Time of Flight (TOF) GC/MSCO PHILIS
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PHILIS Status - CO

• Have Modified PHILIS 1 System
• 1 45 mobile lab RV (PAL)
• 1 53 sample prep trailer (SPA)
• 3 TOF GC/MS systems w/rapid GC
• 3 SPE systems for SVOCs
• Need - Permanent shore power and shelter solution
• Complete lease transactions on warehouse lease
• Support offices, storage area, meeting/training
  rooms
• HVAC renovations as per HS requirements (if
  required)
• Link in with NCERT/NEIC for TOF methodologies
• SLA trailer w/UDA CWA standards secure room
• VOCs sample prep system (PT systems)
• Upgrade of SVOCs sample prep system (PSE systems)
• LIMS upgrade

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PHILIS 2 (PHILBERT) - KY

• Mobile laboratory consortium of 3 vehicles
• Analytical Trailer with 6 GC/MS systems and one
  LC/MS/MS system
• Sample Preparation Trailer for prep. operations
  of soil, water, air, and wipe samples.
• Sample Log-In and Standards (ultra-dilute CWAs)
  Storage trailer
• Anticipated Capacity of 100 200 Toxic
  Industrial Chemical or Chemical Warfare Agent
  samples per 24 hr. period
• Will analyze CWAs and their breakdown products
  using SW 846 methodologies found in Standardized
  Analytical Methods (SAM).
• Delivered April 2008, awaiting renovations to
  current building for shore power and
  warehouse/office space.
• Proposed location for CWA training and
  certification for EPAs Laboratory Response Corps
• Prototype for EPAs future PHILIS systems

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PHILBERT KYSPA and PAL Trailers
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PHILBERT KYSLA Trailer (UD CWA Secure Room)
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PHILBERT - KYLC/MS-MS System
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PHILBERT Status - KY

• Have One Complete PHILIS 2 system in KY
• 1 53 lab trailer (PAL)
• 1 53 sample prep trailer (SPA)
• 1 - 24 bed 5th wheel trailer w/Ford 550 for
  sample log-in (SLA)
• 6 - GC/MS w/FPD
• 1 - LC/MS-MS
• 3 - PSE automated sample prep for SVOCs
• 2 - P T systems for VOCs
• Wireless LIMS
• Need Permanent shore power and shelter solution
• Support offices, storage, meeting/training rooms
• HVAC renovations as per HS requirements (if
  required)
• Link in with Region 5/NHSRC LC/MS-MS methodology
  RD

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PHILIS Mission

• Fill perceived EPAs gaps in the capability and
  capacity to analyze CWA contaminated
  environmental samples on-site
• Provide timely analytical data during the
  Consequence Management Phase of the incident
• Lower overall remediation costs
• Lower analytical costs/ turn-around time for data
• Allow more timely, on-site decisions during the
  characterization and remediation (decon) phases
  of the incident response
• Provide reliable data on known QA/QC integrity
  for the decision makers during the final
  clearance and recovery phases of the incident
• Reduce time to recovery
• Validate SAM (through the ERLN) CWA analytical
  methods

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How ??

• Provide high quality analytical data on-site,
  using validated methods
• Automated and rapid sample prep
• Rapid GC/MS, TOF-GC/MS and LC/MS-MS methods
• MDLs at or below Risk-based clearance levels
• Automated Laboratory Information System (LIMS) to
  track sample flow and produce field data of known
  QA/QC integrity

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Next Steps

• Short Term Inventory, Set up, Power, Shelter and
  Storage
• Convert remaining PHILIS 1 assets to PHILIS 2
• Set up for VOCs/SVOCs methods (8260/8270)
• NELAC certification
• Long Term Develop a strategic plan and timeline
  to address
• - Routine and emergency response use
• - Resources including staffing
• - Training
• Long Range Goal provide mobile laboratory
  platforms for the rapid, reliable on-site
  analysis of TICs and CWAs
• ERLN certification for CWA UDA standards

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PHILIS Mission in NJ

• Integrate with Region 2 and ERT emergency
  response and laboratory programs
• In fixed mode provide surge capacity for
  routine analysis
• In mobile mode provide on-site analysis at
  Superfund sites
• Platform for EPAs Laboratory Response Corps
  training
• Methods development and validation for SAM
  methods
• Integration of PHILIS 2 into ERLN and UDA CWA
  programs
• Dual Use of PHILIS systems
• Prepare for and respond to CWA and TIC/TIMs
  incidents

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Questions

• NDT Director Steve Hawthorn
• PHILIS Operations Manager Larry Kaelin, NDT
• CWA Methods and Validation Terry Smith, OEM
• Kaelin.lawrence_at_epa.gov
• 732-452-6454