DERC Islet

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DERC Islet Cell Core
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Goal of the ICC Facility

• To provide specialized support for
• pancreatic islet isolation
• functional studies and assessment of beta-cells
• animal models and in vivo tests for diabetes and
  autoimmune immunology.

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Services

• Islet isolation (mouse, rat, pig, dog, and human)
• Islet culture and in vitro treatments
• Islet transplantation in animal models
• Induction of diabetes in rodent models (mouse or
  rat)
• Functional studies of beta-cells
• Cellular and Molecular studies of beta-cells
• Tissue sharing
• Immunohistochemical staining of pancreatic tissue
  
• Cellular apoptosis, in situ and quantitative

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Islet Isolation

• Inquiry of the service (including animal
  protocols and current funding)
• Transfer animals to ICC
• Isolation
• Culture and assessment

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Islet/beta cell assessment

• Insulin secretion (static and kinetic)
• Apoptosis studies
• Metabolism assessment (MTT, ATP)
• In vivo testing in animal models
• Cellular interaction studies with other
• cells and molecules.

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Diabetic animal model

• Spontaneous diabetic animals (NOD mice and BB
  rats for IDDM)
• Chemical induced diabetic models (STZ)

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Transplantation animal model

• Pancreatic islet transplant in rodents
• (subrenal capsule, liver, thymus)
• Full skin transplant in rodents
• Bone marrow transplant in rodents
• Adoptive transfer of cells in rodents

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Islet b-cell related molecular biology studies

• Real time PCR / RT-PCR
• Apoptosis detection
• MTT, ATP, cellular proliferation
• in situ RT-PCR
• Immunological studies

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Procedures to Obtain Service

• DERC Membership
• Current funding/grant at UVA
• Approved animal protocol
• Contact ICC in advance
• Research plan and requirement
• Animal Transfer
• Schedule procedure

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Service Charges
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Web site Contact List

• http//healthsystem.virginia.edu/internet/ICC/
• CONTACT US

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Services in development

• Human islet isolation study
• Researcher-funded
• HIC approved study plan
• Collaborative projects
• Viral production gene therapy