Rodent Associated Bartonella in Saskatchewan

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Rodent Associated Bartonella in Saskatchewan

• Claire Jardine
• ADED RoundsNov. 26, 2003

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Outline

• Introduction
• Objectives
• Methods
• Results to date
• Plans for next year
• Summary

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Bartonella

• Gram-negative bacteria
• Parasitize mammalian RBC
• Vector-borne

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Bartonella

• Prior to 1992
• Carrions disease B. bacilliformis
• Trench fever B. quintana
• medical curiosities
• bacteria from the past

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Bartonella

• After 1992
• Urban trench fever
• Cat scratch disease
• Bacillary angiomatosis
• Parinauds oculoglandular syndrome

• Peliosis hepatis
• Uveitis
• Encephalitis
• Endocarditis
• Febrile illness
• Neuroretinitis
• Myocarditis

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Bartonella
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Objectives

• Determine if Bartonella occurs in selected small
  mammals in central Saskatchewan
• Determine which isolates of Bartonella are
  infecting small mammals in central Saskatchewan

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Study Areas

• Ten sites within 50 km radius of Saskatoon

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Culture
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PCR
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(No Transcript)
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Phylogeny
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Plan

• Complete PCR, sequencing work
• Develop phylogeny for all isolates

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Objective

• Investigate the ecology of Bartonella infections
  in RGS

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Methods

• Each month at 5 sites

• Trap
• Collect blood

• Mark
• Release

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Results
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Plans

• Demographics
• Follow individual animals
• Relate Bartonella prevalence to flea prevalence
  and load
• Determine the effect of experimentally reducing
  flea loads on Bartonella

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Summary

• Bartonella occurs at high prevalence levels in
  small mammals in Saskatchewan
• New strains associated with Richardsons ground
  squirrels
• Plan
• Ecology
• Fleas

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• The overall host-species diversity of Bartonella
  attests to the ability of members of this genus
  to adapt to a wide range of vertebrate hosts.
  This observation suggests that various members of
  the genus, not currently associated with human
  disease, are likely to continue to evolve to be
  sources of zoonotic and perhaps emergent human
  disease.
• Karem et al. 2000

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Acknowledgements

• Funding/Support
• MRC, WHF, MSFHR
• CDC, Fort Collins
• National Microbiology Lab, Winnipeg
• CCWHC/WCVM
• CWS

• Committee
• Dr. Leighton
• Dr. Wobeser
• Dr. Chirino
• Dr. Artsob
• Dr. Bollinger
• Dr. Jackson

Special Thanks Dr. Kosoy from the CDC, Dr.
Appleyard and the PCR lab, Dr. Chirinos lab, and
D. McColl from Health Canada
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References

• Anderson B.B. and M.A. Neuman. 1997. Bartonella
  spp. as Emerging Human Pathogens. Clinical
  Microbiology Reviews 10(2)203-219.
• Breitschwerdt E.B. and D.L. Kordick. 2000.
  Bartonella Infection in Animals Carriership,
  Reservoir Potential, Pathogenicity, and Zoonotic
  Potential for Human Infection. Clinical
  Microbiology Reviews 13(3)428-438.
• Kosoy M. et al. 2003. Bartonella strains from
  Ground Squirrels are Identical to Bartonella
  washoensis Isolated from a Human Patient.
  Journal of Clinical Microbiology 41(2)645- 650.