Arbovirus Surveillance: Laboratory Tests

1
Arbovirus SurveillanceLaboratory Tests

• Utah West Nile Virus Conference
• February 9, 2004
• Dr. June Inez Pounder
• Utah Department of Health Laboratory

2
Surveillance Lab Tests 2003

• Human- WNV IgM ELISA
• 36 Utah residents tested
• 1 Uintah County 9/18
• 4 Travel associated
• More tested for test validation

3
Surveillance-Lab Tests 2003

• Mosquito-Real-time PCR
• 1945 pools total
• 100 Culex tarsalis, Culex pipiens
• 2 positive C. tarsalis
• Uintah, Utah County 8/11
• Culex erythorthorax in Moab
• activity no target species

4
Surveillance-Lab Tests 2003

• Avian-swabs and tissue by RT-PCR
• 162 samples total
• 126 oral cavity swabs UDOH
• 11 tissue samples UVDL
• 24 live swabs Tracy Aviary-Cornell University
• 2 Grand County
• Crow 9/10
• Magpie 10/9

5
Surveillance-Lab Tests 2003

• Sentinel Chickens-IgM ELISA
• 280 birds 16 weekly bleeds UVDL
• 9 seroconversions WNV
• Carbon County 2 birds 8/4 2 birds 9/2
• Emery County 4 birds 8/18
• Duchesne County 1 bird 9/8

6
Surveillance- Lab Tests 2003

• Equine-WNV IgM ELISA
• 99 total UVDL
• 35 6 counties
• Duchesne 3 Uintah 16
• Emery 8 Wayne 2
• Millard 4 Sanpete 2

7
Surveillance-Lab Tests 2004

• Human
• WNV IgM ELISA-meningoencephalitis
• WNV IgM ELISA-confirm Commercial lab ()
• SLE IgM ELISA
• WNV PCR immunocompromised
• Mosquito
• Culex tarsalis, C. pipiens all tested
• C. erythrothorax-activity in area, special

8
Surveillance-Lab Tests 2004

• Birds
• Oral swabs
• Activity established/cease testing
• Sentinel Chicken Serology
• Increased flocks
• Replace seroconverted birds