Physiological Roles of the Endosperm in Arabidopsis seed germination

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Physiological Roles of the Endosperm in
Arabidopsis Seed Germination
Jessica Hewitt Oregon State University Dept.
Biochemistry and Biophysics
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Mechanism of Germination
Endosperm
Embryo
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Embryo and Endosperm Tissues
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Germination
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Lag Phase Between Testa and Endosperm Rupture
Liu P-P, Homrichhausen TM, Hewitt JR, Nonogaki H,
unpublished results
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Seed Germination Associated Genes
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Insertion Site
Chromosome V
26119K
26126K
20K
21K
22K
23K
24K
25K
At5g65310 Homeobox-leucine zipper protein
At5g65320 Basic helix-loop-helix (bHLH) family
protein
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RNA Expression Analysis of bHLH and LZ
bHLH
LZ
gDNA
RT PCR
gDNA
RT PCR
1.2 kb
1.0 kb
0.8 kb
0.5 kb
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Semiquantitative RNA Expression Analysis
24 h
Dry
0 h
6 h
12 h
18 h
0.5 kb
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Effects of Seed Coat Pigment on Germination
Nesi et al., 2000 Plant Cell 12 1863-1878
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Phenotypic Analysis
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Acknowledgments
Kevin Ahern
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Thanks for Listening