More Albert

1
More Albert

• "Great spirits have always found violent
  opposition from mediocrities. The latter cannot
  understand it when a man does not thoughtlessly
  submit to hereditary prejudices but honestly and
  courageously uses his intelligence."
• "The most beautiful thing we can experience is
  the mysterious. It is the source of all true art
  and all science. He to whom this emotion is a
  stranger, who can no longer pause to wonder and
  stand rapt in awe, is as good as dead his eyes
  are closed."

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Quiz

• Draw a schematic of a ramachandren plot for
  glycine explain why the plot take this shape

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X-ray Crystallography

• Solutions to the Phase problem
• Isomorphous replacement
• Difference Patterson maps
• 3 or more needed for excellent resolution
• Anomalous Scattering
• Heavy atoms present in protein
• Effects are small
• SelenoMet substitution
• Molecular Replacement
• Symmetry restriction

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Interpeting Electron Density Maps
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Four helix bundle topologies
Rop dimer PDB 1ROP
Myohemerythrin PDB code 2mhr
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The Greek Key
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Greek Key StructureBeta sandwich (gamma
crystaline)
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Sea Hare Myoglobin
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