Funding Partners

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Funding Partners
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High throughput protein crystallography bending
magnet beamlineBeamline 3-BM1
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Optics design specifications

• Energy range 5 keV 20 keV
• Bandpass Within 15 of the theoretical value
• Divergence at sample lt2 mrad x lt 0.5mrad (HxV)
  (1mrad acceptance)
• Energy repeatability 0.25eV (0.125eV) (1
  hour)
• Harmonic contamination lt0.1
• Beam focus size at sample lt250?m x lt200?m (HxV)
  (FWHM)
• Positional and intensity stability
• 5 ?m RMS position stability over 1s
• 20?m RMS position stability over 2 hours without
  feedback.
• 5?m RMS position stability over 2 hours with
  feedback.
• 2 RMS intensity on 100 ms to 60 s timescale with
  feedback
• Required flux at the sample gt1011 ph/s in 100?m
  x 100?m _at_ 12.6578 keV

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Optical layout model
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End station
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Computing environment

• 40 TB RAID 5 Storage array
• 3 x dual duo-core XEON, dual head processing and
  graphics machines
• Linux operating system
• FireWire hard drive connection (EXT3)
• Dedicated operator interface computer
• Software
• Data processing and strategy
• CCP4 (Mosflm/Scala) HKL2000 (Denzo/Scalepack)
  XDS/XSCALE Web-Ice Lebelit BEST RODDOSE

• Phasing and Refinement
• CCP4 Solve/Resolve SHELX SnB
• SHARP/autoSHARP ARP/wARP PHASES CNS Phaser C
  HOOCH

• Model Building and Molecular Graphics
• O Coot Grasp LIGPLOT MolScript PyMOL RasM
  OL Raster3D USF XtalView

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Laboratory facilities

• Fume hood
• Common cryo protectants
• Acids
• Some buffers
• Some plastic ware

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General equipment

• 1 Small Leika EZ4 sample mounting
  stereo-microscope (LED lighiting)
• 1 Leica MZ6.5 stereo-microscope, 1x 2x lens
  (LED cold lighting)
• Various cryo tools
• Pipettes x3
• Capillaries
• Liquid nitrogen supply (35l)
• Liquid nitrogen crystal storage dewar (34l)
• Liquid nitrogen dispensing (4l)
• Liquid nitrogen dewars

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Beamline user GUI

• Blu-Ice
• Remote access via NoMachine