Evaluating Mastitis and Collecting Milk Samples - PowerPoint PPT Presentation

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Evaluating Mastitis and Collecting Milk Samples

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Evaluating Mastitis and Collecting Milk Samples – PowerPoint PPT presentation

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Title: Evaluating Mastitis and Collecting Milk Samples


1
Evaluating Mastitis and Collecting Milk Samples
2
CMT Test
Subclinical Mastitis
3
CMT Test
Subclinical Mastitis
4
CMT Test
Subclinical Mastitis
5
CMT Test
Subclinical Mastitis
6
CMT Test
Subclinical Mastitis
7
Clinical Mastitis
Mild
  • Score 1 Mild
  • (signs milk)
  • abnormal milk
  • Score 2 Moderate
  • (signs milk gland)
  • abnormal milk
  • swelling, pain
  • Score 3 Severe
  • (signs milk, gland cow)
  • abnormal milk
  • swelling, pain
  • systemic fever,
  • dehydration shock

Moderate
Severe
8
Milk Cultures
  • Infection level in herd
  • Type of infections
  • Source of infections
  • Control and Prevention
  • Treatment Decisions

9
Milk Cultures
1. Clean teat end with alcohol pledget 2. Collect
milk into a sterile vial 3. Keep the vial on a
45o angle 4. Refrigerate (or ice) sample at
collection
10
Mastitis Pathogen Identification Procedure
Laboratory Team 1. Decision maker 2. Daily
culture routine 3. Veterinarian
Loops
Bi-plate
Incubator
Clinical Mastitis Culture Procedure
Gram-negative Infection
37oC
Flood plate for 30 min
Gram-positive infection
Sampling
Decontaminate plates - discard
Collect mastitis milk sample
Streak milk onto agar bi-plate
Incubate plate for 18-24 hours
Identify the infection
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