Prostate Cancer Bone Metastasis: Biology and Targeting

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Prostate Cancer Bone Metastasis Biology and
Targeting PI Leland W. K. Chung, Ph.D. Core
C Pathology and Laboratory Support Core PI
Majd Zayzafoon, M.D., Ph.D.
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Purpose

• Core C provides pathology and laboratory support
  services for all research projects.
• Core C also support a cDNA expression analysis
  technology core.

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Personnel

• Majd Zayzafoon, Director
• [phone]
• Lauren Beck, Manager
• [phone]
• Kaiyu Yuan, Postdoctoral Fellow
• Omar Hameed, Pathologist
• Patty Lott, Chief Histotechnologist
• Dezhi Wang, Histotechnologist

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Statement of Work

• Provide diagnostic expertise in the pathology of
  prostate cancer bone metastases and prostate
  pathology pertaining to all samples and
  experiments.
• Histology Process fresh or frozen tissue,
  routine staining such hematoxylin and eosin or
  specialized such as TRAP.
• Immunohistochemistry

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Statement of Work (Cont)

• In Situ Hybridization
• Laser Capture Microdissection
• Histomorphometry and photography
• cDNA microarray for the expression profiling
  analysis on experimental cell systems and
  microdissected cellular material. This component
  of the core operates at the Fred Hutchinson
  Cancer Research Center in Seattle, Washington,
  under the directorship of Dr. Peter Nelson

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Data Histology
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Data Histomorphometry
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Projects

• December 2005 - July 2006
• 27 projects have been processed by Core C
• 20 completed and sent back to investigators
• 4 completed and not mailed
• 3 in process
• Total 383 samples

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Users

• Takeo Nomura 16 projects
• Tohru Miyagi 5 projects
• Katsumi Shigemura 1 project
• Valerie Odero-Marah 3 projects
• David Martin 2 projects
• Average completion time is about 32 days

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Requests

• HE staining 100
• TRAP staining 52
• Histomorphometry 26.9
• Unstained slides 92.6

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Future project

• Tissue array
• Different Human Prostate cancer cell lines
  injected into murine bone

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Final Thoughts

• Renewal of P 01 is critical.
• How can Core C help?
• Thank you