Olfactory signal transduction in vertebrates

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Olfactory signal transduction in
vertebrates Johannes Reisert Department of
Neuroscience Johns Hopkins University School of
Medicine
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Firestein, Nature 2001 Frings, Cell Mol Life Sci
2001
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Firestein, Nature 2001
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odorant receptor (P2) tau-lacZ
The numbers 1 receptor/cell 1000 receptors
(mouse)
Mombaerts et al., Cell 1996
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Mori, Science 1999
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metal-filled glass electrode
Firestein, Nature 2001
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Extracellular spike recording and the
Electro-OlfactoryGram (EOG)
Duchamp-Viret et al., Science 1999
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Tight-seal voltage clamp technique
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Firestein et al., J Physiol 1993
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Suction pipette technique
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Reisert Matthews, J Physiol 1999
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Reisert Matthews, J Physiol 1999
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Frings, Cell Mol Life Sci 2001
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Lowe Gold, J Physiol 1991
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cAMP production
Sklar et al., J Biol Chem 1986
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Sklar et al., J Biol Chem 1986
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Lowe et al., Chem Senses 1989
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Nakamura Gold, Nature 1987
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NaCl Ca2
NaCl cAMP
Zufall Firestein, J Neurophysiol 1993
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Dzeja et al., EMBO 1999
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NaCl
NaCl Ca2
Kleene Gesteland, J Neurosci 1991
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Kurahashi Yau, Nature 1993
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Lowe Gold, Nature 1993
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Reisert Matthews, J Gen Physiol 1998
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Reisert Matthews, J Gen Physiol 1998
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NaCl
NaCl Ca2 cAMP
Bradley et al., Science 2001
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Kurahashi Menini, Nature 1997
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Leinders-Zufall et al., J Neurosci 1999
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IP3 pathway ?
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Odorants lead to an increase in IP3 in a ciliary
preparation IP3- gated channel has been reported
to be present in cilia Genetically modified
mice lacking ACIII, CNGA2 or Golf are
anosmic IP3 channel density is only 10 of the
CNG channel density
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Reisert Matthews, J Physiol 2001
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Reisert Matthews, J Physiol 2001
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Reisert Matthews, J Physiol 2001
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Reisert Matthews, J Physiol 2001
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cAMP
PDE Adenylyl cyclase

CNG-channels
-
Ca2
modified from Cooper, Mons Karpen, Nature 1995
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