DNA Replication and Repair

1
DNA Replication and Repair
Chapters 8 and 9
2
DNA Replication
3
DNA Polymerization
4
Base Pairing Fidelity
5
rNTPs Do Not Work
6
DNA Polymerase
7
Divalent Cations are Required
8
DNA Polymerase Binds Template and Free dNTP
9
Processive Replication
10
Proof Reading Accompanies Replication
11
DNA Replication is Uncommonly Accurate
12
The Replication Fork
13
Replacing RNA Primers
14
DNA Helicase Separates Strands
15
Topoisomerase at the Replication Fork
16
Various DNA Polymerases
17
DNA Polymerase Switching
18
Sliding DNA Clamp
19
Sliding DNA Clamp Increases Processivity
20
Trombone Model of DNA Replication in E. coli I
21
Trombone Model of DNA Replication in E. coli II
22
Trombone Model of DNA Replication in E. coli III
23
Trombone Model of DNA Replication in E. coli IV
24
Trombone Model of DNA Replication in E. coli V
25
Helicase Binds to DNA Polymerase III
26
Initiator Proteins at ORI
27
Initiation in E. coli
28
Inactivation After Replication
29
Prereplicative Complex
30
Activation Leads to Assembly
31
Cdk Activity and Cell Cycle Progression
32
Replicating Ends of Linear Chomosomes
33
Protein Priming at the Ends
34
Telomerase ReplicatesTelomeres
35
Telomerase Extends 3 End
36
Base Substitutions
Purine to purine is a transitionPurine to
pyrmidine is a transversion
37
Mutations Can be Fixed
38
Mismatch Repair
39
Dam Methylation
40
Repair is Directional
41
Ames Test
42
Thymidine Dimers
43
Base Analogs Cause Mutations
44
Intercalating Agents Cause Mutations
45
Photoreactivation
Fake and bake?
46
Methyl Transferase Repairs Gs
47
Excision of Uracil from DNA
48
Removal of oxoG by DNA Glycosylase
49
Nucleotide Excision-Repair
50
Repair Coupled to Transcription
51
Translesion DNA Synthesis