Concepts

1
Concept Map
A technique to connect ideas or concepts together
in a way that is meaningful to you.
Concepts Activity Assay Protein
Function PCR DNA Pure Protein Cloned
Gene Recombinant protein Plasmid SDS-PAGE Size
exclusion chromatography Radioisotopes
2
Concept Map
Concepts Activity Assay Protein
Function PCR DNA Pure Protein Cloned
Gene Recombinant protein Plasmid SDS-PAGE Size
exclusion chromatography Radioisotopes
To generate
PCR
DNA
3
Concept Map
Concepts Activity Assay Protein
Function PCR DNA Pure Protein Cloned
Gene Recombinant protein Plasmid SDS-PAGE Size
exclusion chromatography Radioisotopes
Primers
DNA template
requires
requires
To generate
PCR
DNA
4
Concept Map
Concepts Activity Assay Protein
Function PCR DNA Pure Protein Cloned
Gene Recombinant protein Plasmid SDS-PAGE Size
exclusion chromatography Radioisotopes
Should not be found in
Restriction sites
Designed into
Bind to
Primers
DNA template
requires
requires
To generate
PCR
DNA
5
Concept Map
Concepts Activity Assay Protein
Function PCR DNA Pure Protein Cloned
Gene Recombinant protein Plasmid SDS-PAGE Size
exclusion chromatography Radioisotopes
Cloned Gene
To generate
Restriction sites
Should not be found in
Plasmid
Designed into
Bind to
into
Primers
DNA template
Designed with
In-Frame start codon (ATG)
requires
Ligated
requires
To generate
PCR
DNA
6
Anne Lynns Concept Map
Protein Purification
Protein Function
Lysozyme Assay
Is a type of
Included volume
Is a step in
Is a step in
contains
Studied by
Results in
Used to develop
Has a
Size Exclusion Chromatography
Activity Assay
Affinity Chromatography
Total volume
Spectrophotometer
Is a type of
Subjected to
Used to detect
contains
Requires
SDS-PAGE
Recombinant protein
Poly-His
Void volume
b-particles
Can be used to label
Pure Protein
Bradford Assay
emit
Used to detect
Radioisotopes
To express
emit
a-particles
Are a Type of
Put into correct type of
Expression system
Prokaryotic Cells
Have a
Cloned Gene
Are commonly used
Are a Type of
Can be transferred by baculovirus or transfected
into
To generate
E. coli
Should not be found in
Eukaryotic Cells
Restriction sites
Plasmid
Are commonly used
Half-life (t1/2)
Are a Type of
Designed into
into
mammalian cell lines
Insect cell lines
Bind to
Primers
DNA template
Designed with
In-Frame start codon (ATG)
Can be transferred by adenovirus or transfected
into
requires
Ligated
requires
To generate
PCR
DNA
Taq polymerase
requires