Trust is good, control is better!

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Trust is good, control is better!

• Matthias Gessner
• Baxter AG
• Vienna, Austria

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Background

• 71 HCV and 59 HIV commercially available
  quantified virus preparations were obtained from
  various vendors and tested with HIQ-PCR
• Testing in various dilutions by HIQ-PCR in frame
  of performance study
• 11 samples (8) gave unexpected negative results
  in the test, i.e. were not detected in spite of a
  nominally high concentration in the sample
• These samples were sent to the Inst. of Virology,
  Medical Univ. of Vienna for independent
  quantitative testing
• Cobas Amplicor HCV Monitor v2.0
• Cobas Amplicor HIV-1 Monitor v1.5

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Viral load according to
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Conclusion

• Specimens yielding negative or unexpectedly low
  results showed gross deviation between their
  stated and actual viral load when re-quantified
• Since only samples with very obvious deviations
  were re-tested, the percentage of commercially
  available samples with deviating concentration
  values may be higher
• Quantitative information provided with
  commercially available viral specimens, such as
• Clinical samples
• Viral preparations
• Seroconversion panels
• may by misleading and thus, needs to be regarded
  with caution.

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