Assembly and analysis of DNA

1
Assembly and analysis of DNA
2
What is a sequence assembly?

• Putting together pieces of the DNA that was
  previously sequenced.
• Also means align, edit and correct errors in
  order to obtain a consensus sequence.

3
Some key features of successful editors

• Use of color or symbols to illustrate key
  features (different bases, conserved regions)
• Ability to see the traces files
• Ability to display the complementary strand
• Possibility to edit sequences
• And others

4
How to calculate the average coverage of the
consensus?

• 2 ways to do that
• ( seq reactions X of bases)/gene or genome
  size
• Ex 20 seq.reactions X 500bases/ 2,000 5
• - Select several parts of the consensus sequence
  (ex beginning, middle and end) and see how many
  seq. reactions was needed and take the mean of
  those.