cellspecific

1
Experiments / perturbationsStimulation with
different combinations and concentrations of
ligands // RNAi knockdowns
42 different ligands
cytoplasm
5 different Type II receptors
7 different Type I receptors
Smad1/2
nucleus
activated Smad complex
co-activator or co-repressor
cell-specific DNA-binding cofactors
Smad1/2-responsive genes
Smad4
Assay 2 Transcriptional assay using
Smad-responsive promoter elements No temporal
resolution (red/yellow) mRFP1 and Venus
coupled to specific promoters With temporal
resolution (red/green to blue) Nitroreductase
and beta-lactamase
Assay 1 Pathway activation assay by tracking
nuclear translocation of Smad-FP fusions Option 1
(red/green) Option 2 (blue/yellow) mRFP1 or
JRed-Smad1 Cerulean-Smad1 EGFP-Smad2
Venus-Smad2