Apyrase inhibitors affect pollen growth

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Apyrase inhibitors affect pollen growth

• Discovery Lab Lecture
• February 7, 2008

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Apyrase inhibitors were discovered in a screen of
9,000 small organic molecules

• Assay tested whether randomly selected organic
  compounds could block the ability of potato
  apyrase to remove phosphates from ATP
• Only 17 compounds were found to be effective
  inhibitors
• These inhibitors differed in their relative
  specificity
• These were the first compounds found to be very
  effective inhibitors of apyrase activity

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Inhibitors NGXT 191, NGXT 1913 and 4 differ in
their ability to inhibit other phosphatases
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NGXT 191 and Inhibitor 4 simultaneously inhibit
pollen tube growth and raise the eATP
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Sample results from last week

• Low 0.32 vs 0.46 0.03 vs 0.12
• 0.36 vs. 0.15 2.21 vs. 0.56
• High 0.14 vs. 0.15 0.15 vs. 0.15
• 0.08 vs. 0.07 0.07 vs. 0.01

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This week

• Review data from last week v
• Training on how to make bar graphs (for next
  weeks presentations and reports).
• Transfer samples planted last week to test media
  (Round 1 of experiments)
• gt low (30 mM) or high (150 mM) GTP?S
• gt inhibitor 2 (various concentrations)
• gt DPI (5 or 10 mM) 30 mM ADP?S
• Then measure effects on root hair growth

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Next week

• Those giving oral reports need to load their
  presentations between 200 and 225 PM on
  Thursday (or send file to Dr. Clark by e-mail
  ask him to load it). NOTE If you have new Vista
  operating system, save your .ppt in old system.
• Those giving written reports, send your file to
  Dr. Clark by 215 on Thursday, and bring a hard
  copy to class.

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Next week
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