Multiparametric flow cytometry of

1

• Multiparametric flow cytometry of
• normal human bone marrow
• analysis and display strategies

GEIL-GTLLF 2008
Part four Lymphocytic maturation
IV.1
2
Display
Backgating
Strategy
IV.2
3
Informative combinations

• Tube 1 CD11b/CD14/CD16/CD45 Tube 9
  CD64/CD24/CD10/CD45
• CD11b/CD16 p. IV.4 CD24/CD10 p. IV.9
• Tube 5 CD38/DR/CD34/CD45 Tube 10
  CD7/CD117/CD34/CD45
• CD38/CD34 p. IV.5 CD7/CD117 p. IV.10
• CD7/CD34 p. IV.11 .
• Tube 6 CD38/CD10/CD19/CD45
• CD38/CD10 p. IV.6 Tube 11
  CD22/CD10/CD20/CD45
• CD22/CD10 p. IV.12
• Tube 8 CD34/CD56/CD19/CD45 CD10/CD20 p.
  IV.13
• CD34/CD19 p. IV.7
• CD56/CD19 p. IV.8

IV.3
4
CD11b/CD16
Gating CD11b
Gating CD16
Lymphober
IV.4
5
CD38/CD34
Gating CD38
Gating CD34
Lymphober
IV.5
6
CD38/CD10
Gating CD38
Gating CD10
Lymphober
IV.6
7
CD34/CD19
Gating CD34
Gating CD19
Lymphober
IV.7
8
CD56/CD19
Gating CD56
Gating CD19
Lymphober
IV.8
9
CD24/CD10
Gating CD24
Gating CD10
Lymphober
IV.9
10
CD7/CD117
Gating CD7
Gating CD117
Lymphober
IV.10
11
CD7/CD34
Gating CD7
Gating CD34
Lymphober
IV.11
12
CD22/CD10
Gating CD22
Gating CD10
Lymphober
IV.12
13
CD10/CD20
Gating CD20
Gating CD10
Lymphober
IV.13