Biological Safety Cabinets

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Biological Safety Cabinets

• Marcy Huey
• August 2009

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Hazard Levels?

• What are you talking about??!!
• Hazard Group 1 2 3 4 (of pathogens)
• Containment Level 1 2 3 4 (of laboratories)
• Class 1 2 3 4 (of Genetic Modification)
• CLASSI II III (of Safety Cabinets)

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The terminology SafetyCabinets

• This is one of the most common mistakes made
• Tissue Culture Hood, Laminar Flow, Glove Box,
  Fume Hood, Hoods, Cabinets, Isolator not all
  exactly the same
• Microbiological Safety Cabinet Class I, II or III
• Also called Horizontal Laminar Flow/ Vertical
  Laminar Flow
• Sometimes called Isolators
• Or Fume Cupboards

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Containment Levelof Laboratories

• CL1-Ill just do it on the bench over there..
• CL2-OK, so Ill have to use the hood.
• CL3-Thats that room along the corridor that
  no-one goes in?
• CL4-Spacesuits?

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Containment Levelof Laboratories

• CL1-easy to clean, impervious resistant
  surfaces, disinfectants to hand,minimise
  aerosols, door shut during work. GLP -lab coats
  and PPE, no mouth pipetting, eating etc etc, hand
  wash sink, safe disposal routes in addition to
  the above.
• CL2-authorised access only, specified
  disinfection procedures, safe storage of
  biological agents, work producing aerosols must
  be in MSC, access to incinerator for some bio
  waste, 24m3 space per person, autoclave, negative
  airflow during work if possible, usually require
  BSC

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Containment Levelof Laboratories

• CL3-Must be maintained at negative pressure,
  sealable for fumigation, extracted air must be
  HEPA filtered.Must be separated from any other
  activities in the building.Secure access list
  of authorised personnel posted on entrance.
  Observation window.Laboratory to contain its own
  equipment, BSC autoclave.Impervious floor as
  well as benches.Written contingency plans in case
  of spillage. Written training records. in
  addition to the above.UA doesnt currently have
  any CL3 labs.
• CL4-The University has no CL4 labs.

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BSC vs Fume Hood

• Biological Safety Cabinets (BSC) provide
• Protection for the user and the product
• HEPA filtration of air into cabinet and exhaust
  air
• Fume hoods provide
• Removal of contaminated air
• Protection for the user from chemicals

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BSCs vs Fume Hoods

• Biological Safety Cabinets provide user
• and product protection via HEPA
• filtered air circulation.
• Fume Hoods provide user protection
• via removal of contaminated air to external
• exhaust.

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Biological Safety Cabinets

• Must be properly maintained and regularly
  serviced
• Must be certified annually and when new, moved,
  or repaired
• Must be used in conjunction with good
  microbiological techniques
• Provide protection for personnel, product, and
  environment

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General Cabinet Information

• Types of Biological Safety Cabinets
• Choose the correct Biological Safety Cabinet for
  your experiments.
• Contact the Department of Environmental Health
  and Safety before purchasing a new cabinet or
  beginning a new project for help in selecting the
  appropriate cabinet.
• Biosafety in Microbiological and Biomedical
  Laboratories http//www.cdc.gov/od/ohs/biosfty/bmb
  l5/bmbl5toc.htm

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