Characterization of Gene Expression by Intradermal Administration

1
Modifying the Properties of DCs as Innovative
Strategies to Enhance DNA Vaccine Potency
Schematic diagram to show DNA vaccination via
gene gun
Gene Gun
Characterization of Gene Expression by
Intradermal Administration
pcDNA3-Luc
pcDNA3
Strategies to Enhance DNA Vaccine Potency

• Employment of intracellular sorting signals to
  improve antigen processing through MHC class I
  and II pathways.
• Employment of intercellular spreading strategies
  to increase the number of antigen presenting
  cells that present antigens encoded by DNA
  vaccines.
• Employment of Anti-apoptotic strategies to
  prolong life span of antigen presenting cells
  that present antigens encoded by DNA vaccines

One hour after DNA vaccination